Spectrochim Acta A Mol Biomol Spectrosc
Key Laboratory of Chemo/Biosensing and Detection, Xuchang University, Xuchang 461000, PR China; College of Chemical and Materials Engineering, Xuchang University, Xuchang 461000, PR China; College of Chemistry, Zhengzhou University, Zhengzhou 450052, PR China. Electronic address:
Published: November 2024
As one of endogenous reactive oxygen species (ROS), peroxynitrite (ONOO) performs various functions in both pathological and physiological mechanisms. In this work, an optical and near-infrared (NIR) fluorescent probe (NX), which based on 3-dihydro-1H-xanthene and 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) group was designed and prepared to detect ONOO. This probe revealed an obvious optical and a fluorescent response when ONOO was present and it exhibited higher selectivity over other ROS. Especially, the dual NIR fluorescence changes at 660 and 800 nm allowed quantitative detection of ONOO in the range of 15-40 μM, and the detection limit was 82 nM. Finally, the probe was effectively employed to visualize exogenous and endogenous ONOO in HepG2 cells and zebrafish, respectively. All the results indicated the dual NIR-channel probe could serve as a potent detecting tools in studying ONOO in vitro and in vivo.
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http://dx.doi.org/10.1016/j.saa.2024.124665 | DOI Listing |
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