AI Article Synopsis
- The study investigated how the bacteria Herbaspirillum seropedicae SmR1 adapts its metabolism under different nitrogen conditions and the role of the NtrC protein in this process.
- Under ammonium limitation, the bacteria activate specific transport systems and metabolic pathways to utilize available nitrogen while downregulating energy-related genes.
- The research found that NtrC coordinates transcriptional responses to nitrogen availability, enhancing the bacteria's ability to manage nitrogen and maintain cellular balance.
Article Abstract
The RNA-Seq profiling of Herbaspirillum seropedicae SmR1 wild-type and ntrC mutant was performed under aerobic and three nitrogen conditions (ammonium limitation, ammonium shock, and nitrate shock) to identify the major metabolic pathways modulated by these nitrogen sources and those dependent on NtrC. Under ammonium limitation, H. seropedicae scavenges nitrogen compounds by activating transporter systems and metabolic pathways to utilize different nitrogen sources and by increasing proteolysis, along with genes involved in carbon storage, cell protection, and redox balance, while downregulating those involved in energy metabolism and protein synthesis. Growth on nitrate depends on the narKnirBDHsero_2899nasA operon responding to nitrate and NtrC. Ammonium shock resulted in a higher number of genes differently expressed when compared to nitrate. Our results showed that NtrC activates a network of transcriptional regulators to prepare the cell for nitrogen starvation, and also synchronizes nitrogen metabolism with carbon and redox balance pathways.
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| http://dx.doi.org/10.1007/s00203-024-04044-x | DOI Listing |
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