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Advances in Nucleic Acid Assays for Infectious Disease: The Role of Microfluidic Technology. | LitMetric

Advances in Nucleic Acid Assays for Infectious Disease: The Role of Microfluidic Technology.

Molecules

Engineering Research Center of Optical Instrument and System, The Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China.

Published: May 2024

AI Article Synopsis

  • Microfluidic-based integrated technology systems are improving the speed, accuracy, and portability of detecting pathogens through techniques like RT-PCR, LAMP, and CRISPR.
  • These advancements have successfully identified various pathogens such as COVID-19, Ebola, Zika, and dengue fever, highlighting the importance of integrating traditional molecular biology with cutting-edge technology.
  • Future research aims to enhance sensitivity, simplify testing processes, lower costs, and improve remote data sharing to strengthen global public health surveillance and response capabilities.

Article Abstract

Within the fields of infectious disease diagnostics, microfluidic-based integrated technology systems have become a vital technology in enhancing the rapidity, accuracy, and portability of pathogen detection. These systems synergize microfluidic techniques with advanced molecular biology methods, including reverse transcription polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR), have been successfully used to identify a diverse array of pathogens, including COVID-19, Ebola, Zika, and dengue fever. This review outlines the advances in pathogen detection, attributing them to the integration of microfluidic technology with traditional molecular biology methods and smartphone- and paper-based diagnostic assays. The cutting-edge diagnostic technologies are of critical importance for disease prevention and epidemic surveillance. Looking ahead, research is expected to focus on increasing detection sensitivity, streamlining testing processes, reducing costs, and enhancing the capability for remote data sharing. These improvements aim to achieve broader coverage and quicker response mechanisms, thereby constructing a more robust defense for global public health security.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11173870PMC
http://dx.doi.org/10.3390/molecules29112417DOI Listing

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