AI Article Synopsis
- - The study focuses on analyzing a carbapenem-resistant strain of Proteus mirabilis to understand how it develops resistance to antibiotics through detailed genomic analysis and antibiotic susceptibility testing.
- - Whole-genome sequencing revealed a chromosome with over 29 resistance genes in the strain from a tetanus and pneumonia patient, along with the identification of two significant multidrug-resistant gene regions.
- - Findings suggest that this strain acquires resistance genes through unique mechanisms located on its chromosome, rather than on plasmids, highlighting the need for better control strategies in healthcare environments.
Article Abstract
Objective: This study aims to conduct an in-depth genomic analysis of a carbapenem-resistant Proteus mirabilis strain to uncover the distribution and mechanisms of its resistance genes.
Methods: The research primarily utilized whole-genome sequencing to analyze the genome of the Proteus mirabilis strain. Additionally, antibiotic susceptibility tests were conducted to evaluate the strain's sensitivity to various antibiotics, and related case information was collected to analyze the clinical distribution characteristics of the resistant strain.
Results: Study on bacterial strain WF3430 from a tetanus and pneumonia patient reveals resistance to multiple antibiotics due to extensive use. Whole-genome sequencing exposes a 4,045,480 bp chromosome carrying 29 antibiotic resistance genes. Two multidrug-resistant (MDR) gene regions, resembling Tn6577 and Tn6589, were identified (MDR Region 1: 64.83 Kb, MDR Region 2: 85.64 Kbp). These regions, consist of integrative and conjugative elements (ICE) structures, highlight the intricate multidrug resistance in clinical settings.
Conclusion: This study found that a CR-PMI strain exhibits a unique mechanism for acquiring antimicrobial resistance genes, such as bla, located on the chromosome instead of plasmids. According to the results, there is increasing complexity in the mechanisms of horizontal transmission of resistance, necessitating a comprehensive understanding and implementation of targeted control measures in both hospital and community settings.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11186132 | PMC |
| http://dx.doi.org/10.1186/s12866-024-03365-7 | DOI Listing |
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