AI Article Synopsis
- Identifying and characterizing CD8 T-cells is crucial for understanding their role in fighting viral infections.
- The study discusses a peptide-MHC (pMHC) tetramer approach to identify T-cells specific to certain antigens in both humans and mice.
- This method allows for the measurement and functional analysis of T-cells responding to viral antigens from CMV in human blood and rotavirus in mouse intestinal samples.
Article Abstract
Identification and characterization of CD8 T-cells is important to determine their role in protecting and clearing viral infections. Here we provide details of the peptide-MHC (pMHC) tetramers-based approach to identify antigen-specific T-cells in human and murine samples. This method provides ex vivo quantification and functional characterization of T-cells reactive to specific viral antigens derived from CMV and rotavirus in human blood and in murine intestinal lamina propria samples, respectively.
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| http://dx.doi.org/10.1007/978-1-0716-3890-3_19 | DOI Listing |
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