AI Article Synopsis
- Tobacco mild green mosaic virus (TMGMV)-like nanocarriers were developed to effectively deliver genes to plant cells using a polycationic biopolymer, PAH, which enhanced their charge and loading capacity.
- These TMGMV-PAH nanocarriers were shown to efficiently transport DNA into plant cell protoplasts, achieving a 70% viability rate while expressing green fluorescent protein (GFP).
- The inactivated TMGMV-PAH, or iTMGMV-PAH, were created to prevent infection in plants while still facilitating gene delivery, showcasing their potential use in genetic engineering for plants.
Article Abstract
Tobacco mild green mosaic virus (TMGMV)-like nanocarriers were designed for gene delivery to plant cells. High aspect ratio TMGMVs were coated with a polycationic biopolymer, poly(allylamine) hydrochloride (PAH), to generate highly charged nanomaterials (TMGMV-PAH; 56.20 ± 4.7 mV) that efficiently load (1:6 TMGMV:DNA mass ratio) and deliver single-stranded and plasmid DNA to plant cells. The TMGMV-PAH were taken up through energy-independent mechanisms in protoplasts. TMGMV-PAH delivered a plasmid DNA encoding a green fluorescent protein (GFP) to the protoplast nucleus (70% viability), as evidenced by GFP expression using confocal microscopy and Western blot analysis. TMGMV-PAH were inactivated (iTMGMV-PAH) using UV cross-linking to prevent systemic infection in intact plants. Inactivated iTMGMV-PAH-mediated pDNA delivery and gene expression of GFP was determined using confocal microscopy and RT-qPCR. Virus-like nanocarrier-mediated gene delivery can act as a facile and biocompatible tool for advancing genetic engineering in plants.
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| http://dx.doi.org/10.1021/acs.nanolett.3c04735 | DOI Listing |
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