Endothelial cell responses to fluid shear stress from blood flow are crucial for vascular development, function, and disease. A complex of PECAM-1, VE-cadherin, VEGF receptors (VEGFRs), and Plexin D1 located at cell-cell junctions mediates many of these events. However, available evidence suggests that another mechanosensor upstream of PECAM-1 initiates signaling. Hypothesizing that GPCR and Gα proteins may serve this role, we performed siRNA screening of Gα subunits and found that Gαi2 and Gαq/11 are required for activation of the junctional complex. We then developed a new activation assay, which showed that these G proteins are activated by flow. We next mapped the Gα residues required for activation and developed an affinity purification method that used this information to identify latrophilin-2 (Lphn2/ADGRL2) as the upstream GPCR. Latrophilin-2 is required for all PECAM-1 downstream events tested. In both mice and zebrafish, latrophilin-2 is required for flow-dependent angiogenesis and artery remodeling. Furthermore, endothelial-specific knockout demonstrates that latrophilin plays a role in flow-dependent artery remodeling. Human genetic data reveal a correlation between the latrophilin-2-encoding Adgrl2 gene and cardiovascular disease. Together, these results define a pathway that connects latrophilin-dependent G protein activation to subsequent endothelial signaling, vascular physiology, and disease.
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http://dx.doi.org/10.1038/s44318-024-00142-0 | DOI Listing |
Soft Matter
January 2025
Department of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544, USA.
The capillary break-up of complex fluid filaments occurs in many scientific and industrial applications, particularly in bio-printing where both liquid and polymerized droplets exist in the fluid. The simultaneous presence of fluid and solid particles within a carrier fluid and their interactions lead to deviations in the filament break-up from the well-established capillary breakup dynamics of single-phase liquids. To examine the significance of the dispersed phase and the internal interactions between liquid droplets and solid particles, we prepare emulsions through photopolymerization and conduct experimental investigations into the pinch-off dynamics of fluid filaments, focusing on the impact of varying concentrations of liquid droplets (before polymerization) and polymerized droplets.
View Article and Find Full Text PDFNeurogastroenterol Motil
January 2025
Trisco Foods, Carole Park, Queensland, Australia.
Introduction: Fluid thickeners used in the management of oropharyngeal dysphagia exhibit non-Newtonian shear-thinning rheology, impacting their viscosity during deglutition. This study investigated how the rheological properties of thickened fluids affect pharyngeal swallowing parameters in patients with oropharyngeal motor disorders diagnosed by pharyngeal high-resolution manometry impedance (P-HRM-I).
Methods: Seventy-two patients (18-89 years) referred for P-HRM-I were diagnostically assessed with a 10 mL thin bolus.
Front Bioeng Biotechnol
January 2025
Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
Iliac Vein Compression Syndrome (IVCS) is a common risk factor for deep vein thrombosis in the lower extremities. The objective of this study was to investigate whether employing a porous medium model to simulate the compressed region of an iliac vein could improve the reliability and accuracy of Computational Fluid Dynamics (CFD) analysis outcomes of IVCS. Pre-operative Computed Tomography (CT) scan images of patients with IVCS were utilized to reconstruct models illustrating both the compression and collateral circulation of the iliac vein.
View Article and Find Full Text PDFSci Rep
January 2025
Department of Petroleum Engineering, School of Mining and Geosciences, Nazarbayev University, Astana, Kazakhstan.
Geothermal energy, oil industry, and underground gas storage technology require deep drilling. Although oil-based drilling fluids have been widely used, they cause environmental issues. Environmentally friendly Aphronic fluid has emerged as an alternative to oil-based drilling fluid.
View Article and Find Full Text PDFJ Thorac Dis
December 2024
College of Chemistry and Life Science, Beijing University of Technology, Beijing, China.
Background: Left ventricular assist device (LVAD) has been widely used as an alternative treatment for heart failure, however, aortic regurgitation is a common complication in patients with LVAD support. And the O-A angle (the angle between LVAD outflow graft and the aorta) is considered as a vital factor associated with the function of aortic valve. To date, the biomechanical effect of the O-A angle on the aortic valve remains largely unknown.
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