E3 ligase FBXO22 is not significant for spermatogenesis and male fertility in mice.

Background: F-box-only protein 22 (FBXO22), an important substrate receptor of the SKP1-Cullin-F-box (SCF) ubiquitin ligases, has been reported to be involved in many biological processes, including tumorigenesis, neurological disorders, cellular senescence, and DNA damage. However, the specific role of FBXO22 during spermatogenesis is poorly understood.

Methods: We produced conditional knockout (cKO) and global knockout (KO) mice and assessed their sperm masurements using a computer-assisted sperm analysis (CASA) system. Additionally, we conducted histologic staining and immunostaining to examine the impact of loss on spermatogenesis.

Results: Our results revealed that there were no notable differences in semen quality, fertility test results, or histologic findings in -KO and -cKO mice compared to the control group.

Conclusions: Our study demonstrated that is not significant for spermatogenesis or male fertility in mice. These findings will help researchers avoid redundant efforts and serve as a foundational resource for genetic studies on human fertility.