Quorum quenching mediated biofilm impediment in and by leaf extracts of .

Biofilms are complex communities of microorganisms that cause systemic infections, resistance development and delay in healing wounds. Biofilms can form in various parts of the human body, such as the teeth, lungs, urinary tract, and wounds. Biofilm complicates the effects of antibiotics in treating infections. In search of a cure, a plant-based phyto component was selected for this investigation as an anti-quorum-mediated biofilm restricting agent in Gram-negative and Gram-positive . The bioactive components in (DE) ethyl acetate extract were identified using Gas chromatography and mass spectrometry. The extract was examined for toxicity using 3T3 cell lines and brine shrimp and ascertained to be non-toxic. Violacein was inhibited up to 68.81 % in at 0.6 mg/ml concentration. Hemolysin synthesis impediments in and were 80 % and 51.35 %, respectively, at 0.6 mg/ml of DE extract. At 0.6 mg/ml, EPS was abated by up to 49 % in and 35.26 % in . DE extract prevented biofilm formation in and up to 76.45 % and 58.15 %, respectively, while associated eDNA was suppressed up to 67.50 % and 53.47 % at the respective sub-MIC concentrations. Expression of genes such as , and were dramatically reduced in , while genes such as , and were significantly reduced in . Docking demonstrates that two or more DE molecules bind efficiently to the QS receptors of and . Thus, DE extract can be investigated for therapeutic purposes against pathogenic microorganisms by rendering them less virulent through quorum quenching mediated action.