AI Article Synopsis
- The study synthesized biosynthetic intermediates of the siderophore vibrioferrin (VF) as a mix of stereoisomers, including O-citryl-L-serine, 2-aminoethyl citrate, and alanine-2-amidoethyl citrate.
- The synthesized compounds were tested as substrates in enzyme reactions with recombinant PvsA, PvsB, and PvsE proteins, demonstrating that each enzyme effectively produced VF along the biosynthetic pathway.
- The findings enhance the knowledge of VF biosynthetic enzymes and could aid in creating antimicrobial drugs that target and inhibit these enzymes.
Article Abstract
Biosynthetic intermediates of siderophore vibrioferrin (VF), O-citryl-L-serine, 2-aminoethyl citrate, and alanine-2-amidoethyl citrate were respectively synthesized as a mixture of stereoisomers. These compounds were used as substrates for enzyme reactions using recombinant PvsA, PvsB, and PvsE proteins as corresponding enzyme equivalents. The results of our study show that each enzyme reacts with a respective substrate and produces VF along the proposed biosynthetic pathway. Furthermore, the results of this study will contribute to the understanding of VF biosynthetic enzymes and may help in the development of antimicrobial drugs by inhibiting siderophore biosynthetic enzymes.
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| http://dx.doi.org/10.1248/cpb.c24-00168 | DOI Listing |
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