AI Article Synopsis
- Cytochrome P450 is crucial for insect growth and stress resistance, and its genes, like AccCYP6A13 from Apis cerana cerana, are essential for these functions.
- The expression of AccCYP6A13 varies with age, peaking in adult bees, and is influenced by environmental stress, especially in key body parts like legs, brain, and epidermis.
- Knocking down AccCYP6A13 leads to reduced antioxidant activity and increased pesticide vulnerability, highlighting its importance in development and stress response, which enhances our understanding of bee biology.
Article Abstract
Cytochrome P450 plays a crucial role in regulating insect growth, development, and resisting a variety of stresses. Insect metamorphosis and response to external stress are altered by deleting CYP450 genes. In this study, we identified and analyzed a novel gene of CYP450 family, AccCYP6A13, from Apis cerana cerana, and explored its role in the response of Apis cerana cerana to adverse external stressors. It was found that the expression of AccCYP6A13 was spatiotemporal specificity. The expression level increased with age and reached its highest value in the adult stage. The primarily expressiong location were legs, brain, and epidermis of honeybees. Stress conditions can affect the expression of AccCYP6A13 depending on treatment times. RNA interference experiments have shown that knocking down AccCYP6A13 reduces antioxidant activity and deactivates detoxification enzymes, resulting in oxidative damage accumulation and a decline in detoxification capability in bees, as well as inhibiting numerous antioxidant genes. Additionally, knockdown of the AccCYP6A13 gene in Apis cerana cerana resulted in increased sensitivity to pesticides and increased mortality when treated with neonicotinoid pesticides such as thiamethoxam. AccCYP6A13 overexpression in a prokaryotic system further confirmed its role in resistance to oxidative stress. To summarize, AccCYP6A13 may play an essential role in the normal development and response to environmental stress in Apis cerana cerana. Furthermore, this study contributed to the theoretical understanding of bee resistance biology.
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| http://dx.doi.org/10.1016/j.pestbp.2024.105890 | DOI Listing |
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Article Synopsis
- * A study tested the oral toxicity of five common insecticides on four honey bee species, finding that the managed Asian honey bee (Apis cerana) showed the highest tolerance to these chemicals.
- * The wild species, particularly the giant (A. dorsata) and red dwarf honey bee (A. florea), were more sensitive to the pesticides, emphasizing the need for better pesticide risk assessments and regulations to protect various honey bee species.
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