AI Article Synopsis
- Iron deficiency in horticultural crops like pears leads to poor growth and leaf health, but the role of bHLH transcription factors in iron absorption is not well understood.
- This study identified the bHLH gene PbbHLH155 as crucial for enhancing iron absorption, as its overexpression in Arabidopsis and pear calli resulted in increased resistance to iron deficiency.
- PbbHLH155 activates the expression of two positive regulators, PbFRO2 and PbbHLH38, which improve iron absorption activity, providing insights for future pear breeding efforts.
Article Abstract
Iron (Fe) deficiency is a general stress for many horticulture crops, causing leaf chlorosis and stunted growth. The basic-helix-loop-helix (bHLH) transcription factor (TF) was reported to function in Fe absorption; however, the regulatory mechanism of bHLH genes on iron absorption remains largely unclear in pear. In this study, we found that PbbHLH155 was significantly induced by Fe deficiency. Overexpression of PbbHLH155 in Arabidopsis thaliana and pear calli significantly increases resistance to Fe deficiency. The PbbHLH155-overexpressed Arabidopsis lines exhibited greener leaf color, higher Fe content, stronger Fe chelate reductase (FCR) and root acidification activity. The PbbHLH155 knockout pear calli showed lower Fe content and weaker FCR activity. Interestingly, PbbHLH155 inhibited the expressions of PbFRO2 and PbbHLH38, which were positive regulators in Fe-deficiency responses (FDR). Furthermore, yeast one-hybrid (Y1H) and Dual-Luciferase Reporter (DLR) assays revealed that PbbHLH155 directly binds to the promoters of PbFRO2 and PbbHLH38, thus activating their expression. Overall, our results showed that PbbHLH155 directly promote the expression of PbFRO2 and PbbHLH38 to activate FCR activity for iron absorption. This study provided valuable information for pear breeding.
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| http://dx.doi.org/10.1016/j.plaphy.2024.108786 | DOI Listing |
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PbbHLH155 enhances iron deficiency tolerance in pear by directly activating PbFRO2 and PbbHLH38.
Plant Physiol Biochem
August 2024
School of Horticulture, Anhui Agricultural University, Hefei, 230036, China. Electronic address:
Article Synopsis
- Iron deficiency in horticultural crops like pears leads to poor growth and leaf health, but the role of bHLH transcription factors in iron absorption is not well understood.
- This study identified the bHLH gene PbbHLH155 as crucial for enhancing iron absorption, as its overexpression in Arabidopsis and pear calli resulted in increased resistance to iron deficiency.
- PbbHLH155 activates the expression of two positive regulators, PbFRO2 and PbbHLH38, which improve iron absorption activity, providing insights for future pear breeding efforts.
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