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Cationic lipid nanoparticle-mediated delivery of a Cas9/crRNA ribonucleoprotein complex for transgene-free editing of the citrus plant genome. | LitMetric
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Cationic lipid nanoparticle-mediated delivery of a Cas9/crRNA ribonucleoprotein complex for transgene-free editing of the citrus plant genome.

Lamiaa M Mahmoud Manjul Dutt

Plant Cell Rep

Department of Horticultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, USA.

Published: June 2024

A lipofectamine-mediated transfection protocol for DNA-free genome editing of citrus protoplast cells using a Cas9/gRNA ribonucleoprotein (RNP) complex resulted in the production of transgene free genome edited citrus.

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 Source
http://dx.doi.org/10.1007/s00299-024-03254-3DOI Listing

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