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| http://dx.doi.org/10.1038/s41405-024-00236-1 | DOI Listing |
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PRONAME: a user-friendly pipeline to process long-read nanopore metabarcoding data by generating high-quality consensus sequences.
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Bioengineering Unit, Life Sciences Department, Walloon Agricultural Research Centre, Gembloux, Belgium.
Background: The study of sample taxonomic composition has evolved from direct observations and labor-intensive morphological studies to different DNA sequencing methodologies. Most of these studies leverage the metabarcoding approach, which involves the amplification of a small taxonomically-informative portion of the genome and its subsequent high-throughput sequencing. Recent advances in sequencing technology brought by Oxford Nanopore Technologies have revolutionized the field, enabling portability, affordable cost and long-read sequencing, therefore leading to a significant increase in taxonomic resolution.
View Article and Find Full Text PDFAutomated high-resolution 3D inspection methods for sealant applications in aerospace based on line structured light.
Rev Sci Instrum
January 2025
School of Electronic Engineering, Heilongjiang University, Harbin, HeiLongJiang 150080, China.
Gluing is a critical step in aircraft sealing assembly, with glue profile inspection serving as the final quality assurance measure to ensure consistency and accuracy of the sealant coating, allowing timely detection and correction of defects to maintain assembly integrity and safety. Currently, existing glue inspection systems are limited to basic inspection capabilities, lack result digitization, and exhibit low efficiency. This paper proposes a 3D inspection technology for sealant coating quality based on line-structured light, enabling automated and high-precision inspection of sealant thickness, sealant width, positional accuracy, and overlap joint sealant contour through geometric computation.
View Article and Find Full Text PDFAutomatic calculation method for stenosis ratio based on dialysis access ultrasound image segmentation.
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Department of Echocardiography, Ultrasound Diagnostic Center, The First Hospital of Jilin University, Changchun, China.
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View Article and Find Full Text PDFA Versatile Drift-Free Super-Resolution Imaging Method via Oblique Bright-Field Correlation.
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Department of Bioengineering, Department of Electrical and Computer Engineering, Beckman Institute for Advanced Science and Technology, Cancer Center at Illinois, University of Illinois Urbana-Champaign, Urbana, IL, 61801, USA.
High-resolution optical microscopy, particularly super-resolution localization microscopy, requires precise real-time drift correction to maintain constant focus at nanoscale precision during the prolonged data acquisition. Existing methods, such as fiducial marker tracking, reflection monitoring, and bright-field image correlation, each provide certain advantages but are limited in their broad applicability. In this work, a versatile and robust drift correction technique is presented for single-molecule localization-based super-resolution microscopy.
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ISA Trans
December 2024
College of Automation and Electronic Engineering, Qingdao University of Science and Technology, Qingdao 266061, China. Electronic address:
The primary focus of this article is to explore parameter estimation for time-varying systems affected by colored noise. Based on the attributes of the time-varying system with colored noise under investigation, the original system is separated and two different subsystems are reconstructed. To address the influence of the hidden variables in the system and the time-varying noise signal, we introduce auxiliary models into the reconstructed systems to achieve the separation and synchronization estimation of the time-varying parameters within the system.
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