AI Article Synopsis
- Artemisia argyi essential oil (AAEO) has been used traditionally for asthma treatment, but its specific effects and mechanisms have not been clearly defined until now.
- The study conducted various analyses, including histology and metabolomics, to evaluate how AAEO affects asthma symptoms and lung function in an experimental model.
- Results showed that AAEO significantly reduced inflammation and altered key metabolic pathways, suggesting it helps manage asthma by regulating immune responses and improving lung tissue health.
Article Abstract
Ethnopharmacological Relevance: Artemisia argyi essential oil (AAEO) is a traditional herbal remedy for asthma. However, the potential effect of AAEO on asthma has not been elucidated.
Aim Of The Study: To investigate the protective properties of AAEO upon asthma and elucidate its mechanism.
Materials And Methods: The effects of AAEO in asthma were assessed by histology and biochemical analysis. Then, we integrated real-time reverse transcription-quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, immunohistochemistry and metabolomics analysis to reveal its mechanism.
Results: In vivo, AAEO reduced the counts of white blood cells (WBCs) and cytokines in bronchoalveolar lavage fluid (BALF), ameliorated pathologic alterations in lung tissues, and inhibited secretion of OVA-sIgE and muc5ac. Metabolomics results showed that AAEO can exert therapeutic effects on asthmatic mice by regulating disordered arachidonic acid metabolism and tryptophan metabolism. Further studies shown that AAEO inhibited the expression of 5-LOX and reduced the accumulation of CysLTs in mice. Meanwhile, AAEO promoted the activity of IDO-1, facilitated the conversion of tryptophan to kynurenine, and regulated the imbalance of Treg/Th17 immunity. Immunohistochemical results showed that AAEO promoted the expression of IDO-1. RT-qPCR results showed that AAEO promoted the expression of IL-10 and Foxp3 mRNA, and inhibited the expression of IL-17A and RORγt mRNA, thus regulated the imbalance of Treg/Th17 immunity and exerted its therapeutic effects.
Conclusion: AAEO treatment not only attenuates the clinical symptoms of asthma but is also involved in regulating lung tissue metabolism. The anti-asthmatic activity of AAEO may be achieved by reprogramming 5-LOX-CysLTs and IDO-1-KYN pathways.
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| http://dx.doi.org/10.1016/j.jep.2024.118458 | DOI Listing |
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