Misuse of in microbial enrichment analysis.

A modified new method for microbial enrichment analysis, was incorrectly used in many articles due to a lack of comprehensive and systematic understanding of the original method by the researchers, leading to a serious snowball effect. Here we describe the reasons for the misuse of reporter score and its negative impact on microbial research and hope this comment will facilitate community discussion on the importance of statistical rigor, informing future efforts to enhance reliable and reproducible research.