Plasma protein adsorption on blood-contacting surfaces is the initiating significant event and modulates the subsequent coagulation response. Despite decades of research in this area, Vroman's questions in 1986 "?" and "?" remain unanswered due to the lack of detection techniques with sufficient temporal resolution. In this work, we develop a droplet microfluidic technology to detect protein adsorption sequences on six typical blood-contacting surfaces in milliseconds. Apolipoproteins (Apo) are found to be the first proteins to adsorb onto the surfaces in a plasma droplet, and the specific type of apolipoprotein depends on the surface. Apo CI is the first protein adsorbed on gold, platinum, graphene, stainless steel, and polyvinyl chloride with the adsorption time varying from 0.01 to 1 s, while Apo CIII preferentially reaches the titanium alloy surface within 1 s. Subsequent to the initial adsorption, Apo AI, AII, and other proteins continue to adsorb until albumin arrives. Thus, the adsorption sequence is revealed, and Vroman's questions are answered. Moreover, this finding demonstrates the influence of the initial protein adsorption on subsequent coagulation at the surface, and it offers new insights into the development of anticoagulant surfaces.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.analchem.4c01697 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Sequential Growth of Quantized Peptide Brushes on Colloidal Gold.
Langmuir
January 2025
Department of Materials Science and Engineering, University of Delaware, Newark, Delaware 19716, United States.
We synthesized rigid, macromolecular brushes with well-defined and quantized brush lengths on a gold nanoparticle substrate by using a macromolecular "grafting from" approach. The macromonomers used in these brushes were thiol- and maleimide-functionalized peptide coiled coil "bundlemers" that fold into discrete 4 nm × 2 nm (length × diameter) cylindrical nanoparticles. With each added peptide macromonomer layer, brush thickness increased by approximately the length of a single bundlemer nanoparticle.
View Article and Find Full Text PDFMachine learning models for predicting interaction affinity energy between human serum proteins and hemodialysis membrane materials.
Sci Rep
January 2025
Division of Biomedical Engineering, University of Saskatchewan, 57 Campus Drive, Saskatoon, Saskatchewan, S7N 5A9, Canada.
Membrane incompatibility poses significant health risks, including severe complications and potential fatality. Surface modification of membranes has emerged as a pivotal technology in the membrane industry, aiming to improve the hemocompatibility and performance of dialysis membranes by mitigating undesired membrane-protein interactions, which can lead to fouling and subsequent protein adsorption. Affinity energy, defined as the strength of interaction between membranes and human serum proteins, plays a crucial role in assessing membrane-protein interactions.
View Article and Find Full Text PDFPlasmonic Slippery Surface for Surface-Enhanced Raman Spectroscopy and Protein Adsorption Inhibition.
Anal Chem
January 2025
Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal 576104, India.
Slippery liquid-infused porous surfaces (SLIPSs) are a class of surface that offers low contact angle hysteresis and low tilt angle for water droplet shedding. This property also endows the surface with pinning-free evaporation, which in turn has been exploited for analyte concentration enrichment for Surface Enhanced Raman Spectroscopic applications and antibiofouling. Herein, we demonstrate a facile approach for creating SLIPS with low contact angle hysteresis and low tilt angle for water shedding by coating the equal-volume mixture of polydimethylsiloxane (PDMS) and silicone oil.
View Article and Find Full Text PDFAntifouling Zwitterionic Polymer Coatings for Blood-Bearing Medical Devices.
Langmuir
January 2025
Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, United States.
Blood-bearing medical devices are essential for the delivery of critical care medicine and are often required to function for weeks to months. However, thrombus formation on their surfaces can lead to reduced device function and failure and expose patients to systemic thrombosis risks. While clinical anticoagulants reduce device related thrombosis, they also increase patient bleeding risk.
View Article and Find Full Text PDFDynamic responses and adsorption mechanisms of Chlamydomonas reinhardtii extracellular polymeric substances under Cd, Cu, Pb, and Zn exposure.
Environ Pollut
January 2025
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Extracellular polymeric substances (EPS) can effectively attenuate heavy metal mobility in aquatic ecosystems and reduce metal toxicity to cells. However, a systematic study of microalgae EPS responses and their adsorption behaviors, characteristics, and mechanisms under different heavy metal exposures has not been performed. In this study, EPS extracted from Chlamydomonas reinhardtii CC-125 was analyzed for compositional changes (monosaccharides and proteins) under Cd, Cu, Pb, and Zn treatments.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!