AI Article Synopsis
- This study assessed the effectiveness of nanopore sequencing for early diagnosis of tuberculous meningitis (TBM) using cerebrospinal fluid, comparing it to traditional methods like AFB smear and culture.
- Conducted at a hospital specialized in tuberculosis, the study included 64 adult patients suspected of having TBM between August 2021 and August 2023.
- Results showed that nanopore sequencing had a sensitivity of 77.78% and a negative predictive value of 65.52%, outperforming AFB smear and other conventional tests in diagnosing TBM.
Article Abstract
Objective: This study aimed to evaluate the efficiency of nanopore sequencing for the early diagnosis of tuberculous meningitis (TBM) using cerebrospinal fluid and compared it with acid-fast bacilli (AFB) smear, mycobacterial growth indicator tube culture and Xpert (MTB)/rifampicin (RIF).
Design: Single-centre retrospective study.
Setting: The Tuberculosis Diagnosis and Treatment Center of Zhejiang Chinese and Western Medicine Integrated Hospital.
Participants: We enrolled 64 adult patients with presumptive TBM admitted to our hospital from August 2021 to August 2023.
Methods: We calculated the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of AFB smear, culture, Xpert MTB/RIF and nanopore sequencing to evaluate their diagnostic efficacy compared with a composite reference standard for TBM.
Results: Among these 64 patients, all tested negative for TBM by AFB smear. The sensitivity, specificity, PPV and NPV were 11.11%, 100%, 100% and 32.2% for culture, 13.33%, 100%, 100% and 2.76% for Xpert MTB/RIF, and 77.78%, 100%, 100% and 65.52% for nanopore sequencing, respectively.
Conclusion: The diagnostic accuracy of the nanopore sequencing test was significantly higher than that of conventional testing methods used to detect TBM.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11168130 | PMC |
| http://dx.doi.org/10.1136/bmjopen-2023-080904 | DOI Listing |
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