AI Article Synopsis
- The study focuses on var. chinensis, a plant known for its pharmaceutical and ornamental uses, by analyzing primary and secondary metabolites in different in vitro tissues (callus, adventitious roots, and shoots) using advanced chromatography techniques.
- The researchers optimized in vitro culture methods using hormonal treatments on bulbs to promote growth and metabolite production, with specific concentrations found to be most effective.
- Results indicated that shoots and roots cultivated in a particular nutrient medium yielded the highest levels of metabolites, suggesting that this method could be beneficial for mass-producing valuable compounds from the plant.
Article Abstract
var. chinensis is a perennial monocot plant that is well known for its pharmaceutical and ornamental uses. This study aimed to understand the changes in the primary and secondary metabolites in different in vitro tissues of (callus, adventitious root, and shoot) using high-performance liquid chromatography and gas chromatography time-of-flight mass spectrometry. In addition, to optimize the most efficient in vitro culture methods for primary and secondary metabolite production, bulbs were used as explants and cultivated in Murashige and Skoog (MS) medium containing different hormones at various concentrations. In addition, the present study found suitable hormonal concentrations for callus, adventitious root, and shoot induction and analyzed the primary and secondary metabolites. The MS medium supplemented with 1.0 mg L dicamba, 3.0 mg L indole-3-butyric acid (IBA), and 3.0 mg L 6-benzylaminopurine (BAP) was the most efficient media for callus, adventitious root, and shoot induction in . The tissue induced in this medium was subjected to primary (amines, amino acids, organic acids, sugars, and sugar alcohols) and secondary metabolite (galantamine and phenolic acids) analysis. The shoots and roots showed the highest amounts of metabolites. This study showed that bulb in vitro culture can be an efficient micropropagation method for and the production of primary and secondary metabolites, offering implications for the mass production of primary and secondary metabolite compounds from tissues generated in vitro.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11154942 | PMC |
| http://dx.doi.org/10.1021/acsomega.4c01735 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Untangling areas of improvement in secondary prevention of ischemic stroke in patients with atrial fibrillation.
Sci Rep
December 2024
Health Services Research and Pharmacoepidemiology Unit, Foundation for the Promotion of Health and Biomedical Research of Valencia Region (FISABIO), Avenida Cataluña, 21, 46020, Valencia, Spain.
Improvement of post-stroke outcomes relies on patient adherence and appropriate therapy maintenance by physicians. However, comprehensive evaluation of these factors is often overlooked. This study assesses secondary stroke prevention by differentiating patient adherence to antithrombotic treatments (ATT) from physician-initiated interruptions or switches.
View Article and Find Full Text PDFNormative values of spinal and peripheral proprioception in position sense among healthy adolescents and young adults.
Sci Rep
December 2024
Department of Orthopaedics and Traumatology, The University of Hong Kong, Pok Fu Lam, Hong Kong.
Establishing normative values and understanding how proprioception varies among body parts is crucial. However, the variability across individuals, especially adolescents, makes it difficult to establish norms. This prevents further investigation into classifying patients with abnormal proprioception.
View Article and Find Full Text PDFStudy of the litter in the urban environment as primary and secondary microplastics sources.
Sci Rep
December 2024
Environmental Technologies Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Microplastic is one of the most important environmental challenges of recent decades. Although the abundance of microplastics in water sources and water bodies such as the marine were investigated in many studies, knowing the sources of microplastics requires more studies. In this study, litter was investigated as one of the challenges of urban management and the sources of primary microplastic and secondary microplastic in the urban environment.
View Article and Find Full Text PDFBAY 2413555 is a novel selective and reversible positive allosteric modulator of the type 2 muscarinic acetylcholine (M2) receptor, aimed at enhancing parasympathetic signaling and restoring cardiac autonomic balance for the treatment of heart failure (HF). This study tested the safety, tolerability and pharmacokinetics of this novel therapeutic option. REMOTE-HF was a multicenter, double-blind, randomized, placebo-controlled, phase Ib dose-titration study with two active arms.
View Article and Find Full Text PDFA qualitative study on facilitators and barriers to adoption and implementation of nutrition interventions in higher education settings in Flanders.
Sci Rep
December 2024
Department of Movement and Sport Sciences, Faculty of Physical Education and Physiotherapy, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium.
The transition from secondary school to college or university is a well-known and well-studied risk period for weight and/or fat gain and not meeting the dietary recommendations. Higher education acts as a promising setting to implement nutrition interventions. An important condition for intervention success is that interventions are implemented as intended by the protocol and integrated in the institutional policy.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!