AI Article Synopsis
- The Rice-Vannucci model often leads to significant animal loss and inconsistent results due to high variability in injury among labs.
- This new protocol achieves reliable hypoxic-ischemic brain injury in 10-day-old Wistar rat pups without causing any fatalities.
- Key aspects of the procedure include careful control of timing and temperature during carotid artery ligation and hypoxia exposure to ensure successful outcomes.
Article Abstract
The Rice-Vannucci model in rodent pups is subject to substantial loss of animals, result inconsistency, and high lab-to-lab variability in extent and composition of induced injury. This protocol allows for highly predictable and reproducible hypoxic-ischemic cerebral injury lesions in post-natal day 10 Wistar rat pups with no mortality. We describe steps for common carotid artery ligation, brief post-operative normothermia, exposure to hypoxia, and post-hypoxic normothermia. Precise timing and temperature control in each step are crucial for a successful procedure. For complete details on the use and execution of this protocol, please refer to Hartman et al..
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11217776 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2024.103025 | DOI Listing |
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