AI Article Synopsis
- Researchers found that cells from urine of bladder cancer patients can help study the tumors because of their connection to urine.
- They discovered that urine from bladder cancer patients had more immune cells compared to healthy people's urine.
- These immune cells in urine were very similar to those found in the tumors, suggesting that urine cells could help track tumor changes and might be useful for developing treatments.
Article Abstract
Due to bladder tumors' contact with urine, urine-derived cells (UDCs) may serve as a surrogate for monitoring the tumor microenvironment (TME) in bladder cancer (BC). However, the composition of UDCs and the extent to which they mirror the tumor remain poorly characterized. We generated the first single-cell RNA-sequencing of BC patient UDCs with matched tumor and peripheral blood mononuclear cells (PBMC). BC urine was more cellular than healthy donor (HD) urine, containing multiple immune populations including myeloid cells, CD4+ and CD8+ T cells, natural killer (NK) cells, B cells, and dendritic cells (DCs) in addition to tumor and stromal cells. Immune UDCs were transcriptionally more similar to tumor than blood. UDCs encompassed cytotoxic and activated CD4+ T cells, exhausted and tissue-resident memory CD8+ T cells, macrophages, germinal-center-like B cells, tissue-resident and adaptive NK cells, and regulatory DCs found in tumor but lacking or absent in blood. Our findings suggest BC UDCs may be surrogates for the TME and serve as therapeutic biomarkers.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11157455 | PMC |
| http://dx.doi.org/10.1084/jem.20240045 | DOI Listing |
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