anticancer, antioxidant and antibacterial activities of crude extract prepared from habited in Jeddah, Saudi Arabia.

The recent study purposes to evaluate the biological activities of gathered from Jeddah coastal area, Saudi Arabia, with respect to its phytochemical components. Our results indicated that the values ​​of moisture content, ash, total organic matter, total proteins, total lipids and total carbohydrates were 34.25 ± 5.6 %, 40.70 ± 2.3 %, 25.05 ± 1.73 %, 14.39 ± 0.8 %, 4.86 ± 6.9 % and 2.81 ± 1.4 %, respectively. The data also showed that the total phenols and flavonoids were 345.04 ± 1.50 and 320.67 ± 0.92 mg/g in the dried sample, respectively. Furthermore, four compounds were detected by HPLC at very low concentrations (quinic acid, ellagic acid, cinnamic acid, and phenanthrene) and flavonoids data confirmed the presence of apeginin, rudin, diosmin, and quercilin at high concentrations of 141.26, 11.42, 121.75, and 145.28. mg/g, respectively. The crude extract of exhibited cytotoxicity toward hepatocellular carcinoma cells (HepG-2 cell line) using an MTT assay with concentration range between 2 and 500 µg/mL for 48 h with IC 40.02 ± 3.94 µg/mL. Evidently, the extract had Hepatoprotective activity with IC = 447.31 ± 14.59 μg/mL. The IC activity of a crude methanol extract of was compared with that of an antioxidant drug (Torolox). The value (98.82 ± 1.30 μg/mL) was recorded close to Torolox (62.4 ± 0.70 μg/mL). This extract also possessed moderate antibacterial activity with inhibition zones ranging between 10 mm against to 16 mm against .