Investigating the potential of human cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) in in vitro heart models is essential to develop cardiac regenerative medicine. iPSC-CMs are immature with a fetal-like phenotype relative to cardiomyocytes in vivo. Literature indicates methods for enhancing the structural maturity of iPSC-CMs. Among these strategies, nanofibrous scaffolds offer more accurate mimicry of the functioning of cardiac tissue structures in the human body. However, further research is needed on the use of nanofibrous mats to understand their effects on iPSC-CMs. Our research aimed to evaluate the suitability of poly(ε-caprolactone) (PCL) and polyurethane (PU) nanofibrous mats with different elasticities as materials for the maturation of iPSC-CMs. Analysis of cell morphology and orientation and the expression levels of selected genes and proteins were performed to determine the effect of the type of nanofibrous mats on the maturation of iPSC-CMs after long-term (10-day) culture. Understanding the impact of 3D structural properties in in vitro cardiac models on induced pluripotent stem cell-derived cardiomyocyte maturation is crucial for advancing cardiac tissue engineering and regenerative medicine because it can help optimize conditions for obtaining more mature and functional human cardiomyocytes.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11153585 | PMC |
| http://dx.doi.org/10.1038/s41598-024-63905-z | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Synergy of Hydrophilic Properties and Antibacterial Inhibition in Polyvinyl Alcohol Nanofibrous Mats Loaded With Croton Bonplandianum Baill Leaf Extract.
Biopolymers
January 2025
Department of Textile Engineering, Dhaka University of Engineering and Technology, Gazipur, Dhaka, Bangladesh.
The antibacterial nanofibrous mat is crucial in biomedicine as it enhances infection control, expedites wound healing, and mitigates health hazards by decreasing antibiotic usage. A novel synergistic antibacterial and hydrophilic nanofibrous mat successfully fabricated by solution electrospinning from polyvinyl alcohol (PVA) incorporated Croton bonplandianum Baill (CBB) leaves extract. Antioxidant-enriched leaf extract of the CBB plant was integrated with PVA in varying proportions of 30% (CBB-30), 40% (CBB-40), and 50% (CBB-50) to manufacture antibacterial nanofibrous mat.
View Article and Find Full Text PDFAlginate/gelatin blend fibers for functional high-performance air filtration applications.
Int J Biol Macromol
December 2024
Department of Textile Engineering, Istanbul Technical University, Istanbul, Turkey. Electronic address:
Currently, the primary composition of fibrous filter materials predominantly relies on synthetic polymers derived from petroleum. The utilization of these polymers, as well as their production process, has a negative impact on the environment. Consequently, the adoption of air filter media fabricated from natural fibers would yield significant environmental benefits.
View Article and Find Full Text PDFMetal nanoparticles incorporated chitosan-based electrospun nanofibre mats for wound dressing applications: A review.
Int J Biol Macromol
December 2024
Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering and Technology, University of Dhaka, Dhaka 1000, Bangladesh.
Wound healing is a dynamic physiological process essential for regenerating skin and maintaining coherence in hypodermic tissues. Chitosan-based electrospun nanofibre wound dressings show great promise for expediting the integration of skin and tissues due to their nano-topographic, biodegradable, biocompatible, and antimicrobial properties. However, their moderate bactericidal efficacy and limited mechanical strength hinder their widespread clinical application.
View Article and Find Full Text PDFEnhancement of 5-Fluorouracil Drug Delivery in a Graphene Oxide Containing Electrospun Chitosan/Polyvinylpyrrolidone Construct.
Materials (Basel)
October 2024
Nanotechnology and Bio-Engineering Research Group, Health and Biomedical (HEAL) Strategic Research Centre, Atlantic Technological University, Ash Lane, F91 YW50 Sligo, Ireland.
Electrospun nanofibrous mats, consisting of chitosan (CS) and polyvinylpyrrolidone (PVP), were constructed with the addition of graphene oxide (GO) for enhancement of delivery of the 5-Fluorouracil (5-Fu) chemotherapy drug. Upon studying the range of GO concentrations in CS/PVP, the concentration of 0.2% / GO was chosen for inclusion in the drug delivery model.
View Article and Find Full Text PDFA Bottom-Up Approach to Assemble Cell-Laden Biomineralized Nanofiber Mats into 3D Multilayer Periosteum Mimics for Bone Regeneration.
Nano Lett
November 2024
National Engineering Research Center for Biomaterials, Department of Biomedical Engineering, Sichuan University, Chengdu 610064, China.
The creation of complex multilayer periosteal graft structures is challenging. This study introduced a novel bottom-up approach to assemble cell-laden nanofiber mats into a three-dimensional (3D) multilayer periosteum mimic, successfully replicating the hierarchical complexity of the natural periosteum. These nanofiber mats, which were fabricated by electrospinning, surface modification, and stimulated body fluid (SBF) immersion, are composed of nanoscale polycaprolactone (PCL) fibers coated with a mineralized collagen layer along the fiber orientation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!