AI Article Synopsis
- Superresolution microscopy (SR microscopy) is crucial for studying protein-protein interactions (PPIs) within cells, but effective technologies for this purpose have been lacking.
- The authors developed a new fluorescent probe called split-Dendra2, which significantly enhances the detection of PPIs, allowing for high-resolution imaging.
- Split-Dendra2 has been successfully used to visualize PPIs in small subcellular structures, like clathrin-coated pits and focal adhesions, which were previously difficult to observe, thus providing new insights into cellular functions at the nanoscale.
Article Abstract
Superresolution microscopy (SR microscopy) of protein-protein interactions (PPIs) occurring in subcellular structures is essential for understanding cellular functions. However, a powerful and useful technology for SR microscopy of PPIs remains elusive. Here, we develop a highly efficient photoconvertible fluorescent probe, named split-Dendra2, for SR microscopy of PPIs in the cell. We found that split-Dendra2 enables a highly efficient detection of PPIs, making it possible to perform SR microscopy of PPIs with high spatial resolution and high image reconstruction fidelity. We demonstrate the utility of split-Dendra2 by visualizing PPIs occurring in small subcellular structures at the superresolution, such as clathrin-coated pits and focal adhesions, which cannot be visualized by the existing tools. Split-Dendra2 offers a powerful and useful tool that greatly expands the possibility of SR microscopy and can contribute to revealing the function of PPIs at the nanoscale resolution.
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| http://dx.doi.org/10.1021/acschembio.4c00075 | DOI Listing |
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