AI Article Synopsis
- * The study presents a protocol for a co-culture assay that allows for the robust growth of primary T-ALL cells in a lab setting using 3D organoids.
- * This method is useful for conducting functional studies and drug testing with patient-derived T-ALL cells, providing insights for better treatments.
Article Abstract
T cell acute lymphoblastic leukemia (T-ALL) is a rare but aggressive hematological cancer that occurs primarily in children and adolescents. Here, we present a protocol for in vitro co-culture assay that enables robust expansion of primary T-ALL cells. We describe steps for seeding T-ALL and stromal cells in 3D organoids and subsequent flow analysis to capture the T-ALL cell growth for long-term culture. This protocol provides a valuable platform for in vitro functional studies and drug screenings using patient-derived cells. For complete details on the use and execution of this protocol, please refer to Rivera et al..
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11179097 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2024.103103 | DOI Listing |
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