AI Article Synopsis
- - Bone marrow mesenchymal stem cells (BMSCs) can turn into cartilage cells, and the long noncoding RNA UCA1 enhances this process by positively influencing chondrogenic differentiation.
- - When UCA1 is reduced or knocked down, it leads to lower expression of markers important for chondrogenic differentiation, indicating its critical role.
- - UCA1 interacts directly with the PARP1 protein, promoting its stability through a process called deubiquitination, which in turn activates the NF-κB signaling pathway involved in the differentiation of MSCs.
Article Abstract
Bone marrow mesenchymal stem cells (BMSCs) possess the potential to differentiate into cartilage cells. Long noncoding RNA (lncRNAs) urothelial carcinoma associated 1 (UCA1) has been confirmed to improve the chondrogenic differentiation of marrow mesenchymal stem cells (MSCs). Herein, we further investigated the effects and underlying mechanisms of these processes. The expression of UCA1 was positively associated with chondrogenic differentiation and the knockdown of UCA1 has been shown to attenuate the expression of chondrogenic markers. RNA pull-down assay and RNA immunoprecipitation showed that UCA1 could directly bind to PARP1 protein. UCA1 could improve PARP1 protein via facilitating USP9X-mediated PARP1 deubiquitination. Then these processes stimulated the NF-κB signaling pathway. In addition, PARP1 was declined in UCA1 knockdown cells, and silencing of PARP1 could diminish the increasing effects of UCA1 on the chondrogenic differentiation from MSCs and signaling pathway activation. Collectively, these outcomes suggest that UCA1 could act as a mediator of PARP1 protein ubiquitination and develop the chondrogenic differentiation of MSCs.
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| http://dx.doi.org/10.1093/stmcls/sxae038 | DOI Listing |
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