AI Article Synopsis
- CTF estimation is crucial for obtaining high-resolution structures in cryo-electron tomography (cryoET), but challenges arise due to low signal quality and varying defocus in images.
- The new software CTFMeasure implements a joint estimation method that enhances accuracy by utilizing Thon-ring signals from the entire tilt series.
- CTFMeasure effectively estimates CTF parameters and tilt angle offsets for each micrograph, showing reliable results in both synthetic and real data tests.
Article Abstract
Contrast transfer function (CTF) estimation is a necessary step in the cryo-electron tomography (cryoET) workflow and essential for high-resolution in situ structural determination. However, the low signal-to-noise ratio and continuous defocus variation in micrographs of cryoET tilt series make accurate CTF estimation challenging. Here, we report a tilt-series-based joint CTF estimation method implemented in the new software CTFMeasure. The joint estimation method combines all Thon-ring signals in a tilt series to improve the estimation accuracy. By using an objective function involving the CTF parameters and geometric parameters of a cryoET tilt series, CTFMeasure can estimate the CTF parameters of each micrograph and the absolute tilt angle offset of the lamellar sample relative to the sample stage plane, which is usually the glancing angle used during focused ion beam (FIB) milling. Tests on both synthetic and experimental data, as well as subtomogram averaging, demonstrated the accurate CTF estimation of cryoET tilt series by CTFMeasure.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.str.2024.05.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!