A forkhead transcription factor contributes to the regulatory differences of pathogenicity in closely related fungal pathogens.

and its sister species are important human fungal pathogens. Despite their phylogenetically close relationship, these two pathogens are greatly different in their clinical characteristics. However, the determinants underlying the regulatory differences of their pathogenicity remain largely unknown. Here, we show that the forkhead transcription factor Hcm1 promotes infection in  but not in . Monitoring and fitness outcomes of multiple clinical isolates from the two pathogens indicates that Hcm1 mediates pathogenicity in through its key involvement in oxidative stress defense. By comparison, Hcm1 is not critical for antioxidation in . Furthermore, we identified , which encodes the antioxidant sulfiredoxin, as a conserved target of Hcm1 in two pathogens. Like , had a greater role in antioxidation in than in . Significantly, overexpression of can largely rescue the defective pathogenicity caused by the absence of Hcm1 in . Conversely, Srx1 is dispensable for virulence in . Overall, our findings demonstrate that the difference in the contribution of the antioxidant sulfiredoxin to oxidative stress defense underlies the Hcm1-mediated regulatory differences of pathogenicity in two closely related pathogens.