AI Article Synopsis

  • E. coli cells were modified to include plasmids with the valS gene from the Clark-Carbon collection.
  • These modified cells showed higher levels of valyl-tRNA synthetase activity but did not produce a corresponding amount of a modifying peptide during T4 phage infection.
  • The study suggests that the activity of valyl-tRNA synthetase can be influenced by the interaction between phage infection and gene expression.

Article Abstract

Escherichia coli cells with plasmids bearing the valS gene were constructed from the Clark-Carbon collection. Their increased valyl-tRNA synthetase (EC 6.1.1.9) activity was not matched by commensurate production of a modifying peptide during T4 infection. Thus, phage vs gene expression is set to modify the normal amount of valyl-tRNA synthetase activity.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC254690PMC
http://dx.doi.org/10.1128/JVI.53.2.702-704.1985DOI Listing

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