In this study, 858 novel long non-coding RNAs (lncRNAs) were predicted as sensitive and resistant strains of Haemonchus contortus to ivermectin. These lncRNAs underwent bioinformatic analysis. In total, 205 lncRNAs significantly differed using log2 (difference multiplicity) > 1 or log2 (difference multiplicity) < - 1 and FDR < 0.05 as the threshold for significant difference analysis. We selected five lncRNAs based on significant differences in expression, cis-regulation, and their association with the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways. These expressions of lncRNAs, namely MSTRG.12610.1, MSTRG.8169.1, MSTRG.6355.1, MSTRG.980.1, and MSTRG.9045.1, were significantly downregulated. These findings were consistent with the results of transcriptomic sequencing. We further investigated the relative expression of target gene mRNAs and the regulation of mRNA and miRNA, starting with lncRNA cis-regulation of mRNA, and constructed a lncRNA-mRNA-miRNA network regulation. After a series of statistical analyses, we finally screened out UGT8, Unc-116, Fer-related kinase-1, GGPP synthase 1, and sart3, which may be involved in developing drug resistance under the regulation of their corresponding lncRNAs. The findings of this study provide a novel direction for future studies on drug resistance targets.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00436-024-08238-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Role of autophagy in resistance to ivermectin in Haemonchus contortus.
Vet Parasitol
January 2025
School of Life Sciences, Ningxia University, Yinchuan 750021, China; Key Lab of Ministry of Education for the Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China. Electronic address:
Haemonchus contortus is a highly pathogenic gastrointestinal nematode that parasitizes the abomasum of cattle, sheep, and other ruminants. Long-term use of large quantities of ivermectin (IVM) to control H. contortus has resulted in the development of drug resistance.
View Article and Find Full Text PDFIvermectin induces oxidative stress and mitochondrial damage in Haemonchus contortus.
Vet Parasitol
January 2025
Key Laboratory of Grass-Feeding Livestock Healthy Breeding and Livestock Product Quality Control, Veterinary Research Institute, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, PR China. Electronic address:
Haemonchus contortus poses a severe threat to livestock, leading to substantial economic losses. The extensive use of ivermectin (IVM), an effective anthelmintic drug, has resulted in the development of resistant strains, reducing the drug's effectiveness and making disease management more challenging. Hence, understanding the underlying mechanisms of IVM resistance is crucial.
View Article and Find Full Text PDFCharacterisation of seryl tRNA synthetase (srs-2) in Haemonchus contortus and Teladorsagia circumcincta.
Exp Parasitol
November 2024
AgResearch Ltd, Private Bag 11-008, Palmerston North, New Zealand.
The aim of the study was to purify and characterise recombinant proteins with the potential as an anti-parasite vaccine. Full-length cDNAs encoding seryl-tRNA synthetase (srs-2) were cloned from Haemonchus contortus (HcSRS-2) and Teladorsagia circumcincta (TcSRS-2). TcSRS-2 and HcSRS-2 cDNA (1458bp) encoded proteins of 486 amino acids, each of which was present as a single band of about 55 kDa on SDS-PAGE.
View Article and Find Full Text PDFThe Mitogenome of the Haecon-5 Strain of and a Comparative Analysis of Its Nucleotide Variation with Other Laboratory Strains.
Int J Mol Sci
August 2024
Department of Biosciences, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, VIC 3010, Australia.
Article Synopsis
- The barber's pole worm is a harmful gastric nematode affecting ruminants, leading to significant economic losses in livestock globally.
- Recent studies have utilized the well-mapped genome of the MHco3(ISE).N1 strain for research, but a genetic comparison with the Haecon-5 strain and others had not been done until now.
- The newly assembled mitochondrial genome of Haecon-5 revealed multiple single nucleotide polymorphisms, indicating notable genetic variations, highlighting the need for further research into their biological implications and nuclear genomic differences.
Chromosome-contiguous genome for the Haecon-5 strain of Haemonchus contortus reveals marked genetic variability and enables the discovery of essential gene candidates.
Int J Parasitol
November 2024
Department of Veterinary Bioscience, Melbourne Veterinary School, Faculty of Science, The University of Melbourne, Parkville, Victoria, Australia. Electronic address:
Millions of livestock animals worldwide are infected with the haematophagous barber's pole worm, Haemonchus contortus, the aetiological agent of haemonchosis. Despite the major significance of this parasite worldwide and its widespread resistance to current treatments, the lack of a high-quality genome for the well-defined strain of this parasite from Australia, called Haecon-5, has constrained research in a number of areas including host-parasite interactions, drug discovery and population genetics. To enable research in these areas, we report here a chromosome-contiguous genome (∼280 Mb) for Haecon-5 with high-quality models for 19,234 protein-coding genes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!