Remorin proteins could be positively related to salt and osmotic stress resistance in rapeseed. Remorins (REMs) play a crucial role in adaptations to adverse environments. However, their roles in abiotic stress and phytohormone responses in oil crops are still largely unknown. In this study, we identified 47 BnaREM genes in the B.napus genome. Phylogenetic relationship and synteny analysis revealed that they were categorized into 5 distinct groups and have gone through 55 segmental duplication events under purifying selection. Gene structure and conserved domains analysis demonstrated that they were highly conserved and all BnaREMs contained a conserved Remorin_C domain, with a variable N-terminal region. Promoter sequence analysis showed that BnaREM gene promoters contained various hormones and stress-related cis-acting elements. Transcriptome data from BrassicaEDB database exhibited that all BnaREMs were ubiquitously expressed in buds, stamens, inflorescences, young leaves, mature leaves, roots, stems, seeds, silique pericarps, embryos and seed coats. The qRT-PCR analysis indicated that most of them were responsive to ABA, salt and osmotic treatments. Further mutant complementary experiments revealed that the expression of BnaREM1.3-4C-1 in the Arabidopsis rem1.3 mutant restored the retarded growth phenotype and the ability to resistance to salt and osmotic stresses. Our findings provide fundamental information on the structure and evolutionary relationship of the BnaREM family genes in rapeseed, and reveal the potential function of BnaREM1.3-4C-1 in stress and hormone response.
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http://dx.doi.org/10.1007/s00299-024-03240-9 | DOI Listing |
Mol Biotechnol
December 2024
Department of Biochemistry, Central University of Punjab, Ghudda, Bathinda, Punjab, 151401, India.
Staphylococcus warneri is a gram-positive mesophilic bacterium, resilient to extreme environmental conditions. To unravel its Osmotic Tolerance Response (OTR), we conducted proteomic and metabolomic analyses under drought (PEG) and salt (NaCl) stresses. Our findings revealed 1340 differentially expressed proteins (DEPs) across all treatments.
View Article and Find Full Text PDF3 Biotech
January 2025
Key Laboratory of Three Gorges Regional Plant Genetics & Germplasm Enhancement (CTGU)/Biotechnology Research Center, Three Gorges University, Yichang, 443000 Hubei China.
Unlabelled: Hexokinases (HXK) not only facilitate carbohydrate metabolism but also play important roles in sugar sensing in higher plants. gene families have been extensively discussed in many plant species; however, comprehensive information regarding in sorghum remains unclear. To address this gap, we identified 7 putative sorghum ( to ), and the features of their conserved domains, gene structure, evolutionary tree, and cis-acting elements were systematically characterized to reveal the evolutionary conservation between different plant species.
View Article and Find Full Text PDFMicrobiome
December 2024
School of Agriculture and Biotechnology, Shenzhen Campus of Sun Yat-Sen University, Shenzhen, 518107, China.
Background: Mangrove plants growing in the high salt environment of coastal intertidal zones colonize a variety of microorganisms in the phyllosphere, which have potential salt-tolerant and growth-promoting effects. However, the characteristics of microbial communities in the phyllosphere of mangrove species with and without salt glands and the differences between them remain unknown, and the exploration and the agricultural utilization of functional microbial resources from the leaves of mangrove plants are insufficient.
Results: In this study, we examined six typical mangrove species to unravel the differences in the diversity and structure of phyllosphere microbial communities between mangrove species with or without salt glands.
Plant Sci
December 2024
Department of Life Science, Sogang University, Seoul 04107, Republic of Korea. Electronic address:
Suberin is an extracellular hydrophobic polymer deposited in seed coats that acts as a barrier to regulate the movement of ions, water, and gases, and protects seeds against pathogens. However, the molecular mechanisms underlying suberin deposition in the seed coat remain unknown. In this study, the in planta role of ATP-binding cassette G23 (ABCG23) was investigated in the Arabidopsis seed coat.
View Article and Find Full Text PDFAdv Sci (Weinh)
December 2024
Aiiso Yufeng Li Family Department of Nanoengineering, University of California San Diego, La Jolla, CA, 92093, USA.
Rechargeable Li-SO batteries offer low-cost, high-energy density benefits and can leverage manufacturing processes for the existing primary version at a commercial scale. However, they have so far only been demonstrated in an "open-system" with continuous gas supply, preventing practical application. Here, the utilization and reversibility of SO along with the lithium stability are addressed, all essential for long-life, high-energy batteries.
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