AI Article Synopsis
- Haloarcula japonica, a highly salt-loving archaeon, synthesizes the carotenoid bacterioruberin (BR), and this study explores its biosynthetic pathway.
- Researchers identified two potential genes (c0507 and d1086) that encode phytoene desaturases via genome analysis and performed functional tests on mutants and complemented strains.
- The results showed that both C0507 and D1086 are bifunctional enzymes with similar functions to phytoene desaturase and 3,4-desaturase, and they work together in the biosynthesis of BR, marking a significant finding in the study of halophilic organisms.
Article Abstract
The extremely halophilic archaeon Haloarcula japonica accumulates the C carotenoid, bacterioruberin (BR). To reveal the BR biosynthetic pathway, unidentified phytoene desaturase candidates were functionally characterized in the present study. Two genes encoding the potential phytoene desaturases, c0507 and d1086, were found from the Ha. japonica genome sequence by a homology search using the Basic Local Align Search Tool. Disruption mutants of c0507 and d1086 and their complemented strains transformed with expression plasmids for c0507 and d1086 were subsequently constructed. High-performance liquid chromatography (HPLC) ana-lyses of carotenoids produced by these strains revealed that C0507 and D1086 were both bifunctional enzymes with the same activities as both phytoene desaturase (CrtI) and 3,4-desaturase (CrtD). C0507 and D1086 complemented each other during BR biosynthesis in Ha. japonica. This is the first study to identify two distinct enzymes with both CrtI and CrtD activities in an extremely halophilic archaeon.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1264/jsme2.ME24004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Two Distinct Enzymes Have Both Phytoene Desaturase and 3,4-Desaturase Activities Involved in Carotenoid Biosynthesis by the Extremely Halophilic Archaeon Haloarcula japonica.
Microbes Environ
May 2024
School of Life Science and Technology, Tokyo Institute of Technology.
Article Synopsis
- Haloarcula japonica, a highly salt-loving archaeon, synthesizes the carotenoid bacterioruberin (BR), and this study explores its biosynthetic pathway.
- Researchers identified two potential genes (c0507 and d1086) that encode phytoene desaturases via genome analysis and performed functional tests on mutants and complemented strains.
- The results showed that both C0507 and D1086 are bifunctional enzymes with similar functions to phytoene desaturase and 3,4-desaturase, and they work together in the biosynthesis of BR, marking a significant finding in the study of halophilic organisms.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!