Differential transcriptional profiles of vagal sensory neurons in female and male mice.

Introduction: Differences in metabolic homeostasis, diabetes, and obesity between males and females are evident in rodents and humans. Vagal sensory neurons in the vagus nerve ganglia innervate a variety of visceral organs and use specialized nerve endings to sense interoceptive signals. This visceral organ-brain axis plays a role in relaying interoceptive signals to higher brain centers, as well as in regulating the vago-vagal reflex. I hypothesized that molecularly distinct populations of vagal sensory neurons would play a role in causing differences in metabolic homeostasis between the sexes.

Methods: SnRNA-Seq was conducted on dissociated cells from the vagus nerve ganglia using the 10X Genomics Chromium platform.

Results: Single-nucleus RNA sequencing analysis of vagal sensory neurons from female and male mice revealed differences in the transcriptional profiles of cells in the vagus nerve ganglia. These differences are linked to the expression of sex-specific genes such as , , and . Among the 13 neuronal clusters, one-fourth of the neurons in male mice were located in the -enriched VN1 and VN8 clusters, which displayed higher enrichment of , , , and genes. In contrast, 70% of the neurons in females were found in -enriched clusters VN4, 6, 7, 10, 11, and 13, which showed enriched genes such as , , , , , , and . Two clusters of satellite cells were identified, one of which contained oligodendrocyte precursor cells in male mice. A small population of cells expressed and , indicating that they are epineural adipocytes.

Discussion: Understanding the physiological implications of distinct transcriptomic profiles in vagal sensory neurons on energy balance and metabolic homeostasis would help develop sex-specific treatments for obesity and metabolic dysregulation.