Dual-Emissive Detection of ATP and Hypochlorite Ions for Monitoring Inflammation-Driven Liver Injury In Vitro and In Vivo.

Reactive oxygen species play a pivotal role in liver disease, contributing to severe liver damage and chronic inflammation. In liver injury driven by inflammation, adenosine-5'-triphosphate (ATP) and hypochlorite ion (ClO) emerge as novel biomarkers, reflecting mitochondrial dysfunction and amplified oxidative stress, respectively. However, the dynamic fluctuations of ATP and ClO in hepatocytes and mouse livers remain unclear, and multidetection techniques for these biomarkers are yet to be developed. This study presents , a dual-channel fluorescent bioprobe capable of synchronously detecting ATP and ClO ions. exhibits excellent selectivity and sensitivity for ATP and ClO ions, demonstrating a dual-channel fluorescence response in a murine hepatocyte cell line. Upon intravenous administration, reveals synchronized ATP depletion and ClO amplification in the livers of mice with experimental metabolic dysfunction-associated steatohepatitis (MASH). Through a comprehensive analysis of the principal mechanism of the developed bioprobe and the verification of its reliable detection ability in both and settings, we propose it as a unique tool for monitoring changes in intracellular ATP and ClO level. These findings underscore its potential for practical image-based monitoring and functional phenotyping of MASH pathogenesis.