AI Article Synopsis
- Mechanotransduction is the process where cells convert mechanical forces into biochemical signals, and a new electrochemical sensor offers real-time, quantitative data on these signals, which have previously been challenging to monitor.
- The study introduces a method using a ferromagnetic micropipette to apply local stretching to a single cell, allowing researchers to track biochemical responses directly within the cell.
- The researchers focused on measuring hydrogen peroxide production in endothelial cells in response to various stretching conditions, demonstrating a novel approach to studying mechanotransduction at the single-cell level.
Article Abstract
Mechanotransduction is the essential process that cells convert mechanical force into biochemical responses, and electrochemical sensor stands out from existing techniques by providing quantitative and real-time information about the biochemical signals during cellular mechanotransduction. However, the intracellular biochemical response evoked by mechanical force has been poorly monitored. In this paper, we report a method to apply local stretch on single cell and simultaneously monitor the ensuing intracellular biochemical signals. Specifically, a ferromagnetic micropipette was fabricated to locally stretch a single cell labeled with FeO nanoparticles under the external magnetic field, and the SiC@Pt nanowire electrode (SiC@Pt NWE) was inserted into the cell to monitor the intracellular hydrogen peroxide (HO) production induced by the local stretch. As a proof of concept, this work quantitatively investigated the elevated amount of HO levels in single endothelial cell under different stretching amplitudes. This work puts forward a new research modality to manipulate and monitor the mechanotransduction at the single-cell level.
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| http://dx.doi.org/10.1007/s00216-024-05348-z | DOI Listing |
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