Anemia is a common and serious health problem, nearly universally diagnosed in preterm infants, and is associated with increased morbidity and mortality worldwide. Red blood cell (RBC) transfusion is a lifesaving and mainstay therapy; however, it has critical adverse effects. One consequence is necrotizing enterocolitis (NEC), an inflammatory bowel necrosis disease in preterm infants. The murine model of phlebotomy-induced anemia and RBC transfusion-associated NEC enables a detailed study of the molecular mechanisms underlying these morbidities and the evaluation of potential new therapeutic strategies. This protocol describes a detailed procedure for obtaining murine pups with phlebotomy-induced anemia and delivering an RBC transfusion that develops NEC.
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| http://dx.doi.org/10.21769/BioProtoc.4993 | DOI Listing |
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- In males, while EC levels did not correlate with age, they showed a negative trend with HbA1c; in females, EC levels positively correlated with age but not with HbA1c.
- Notably, menstruating females had higher EC levels compared to non-menstruating ones, yet there were no significant differences in HbA1c levels, suggesting a potential age-related discrepancy in females after puberty.
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