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A high-throughput cell-based screening method for Zika virus protease inhibitor discovery. | LitMetric

A high-throughput cell-based screening method for Zika virus protease inhibitor discovery.

SLAS Discov

Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, Singapore; NTU Institute of Structural Biology, Nanyang Technological University, Singapore, Singapore; National Centre for Infectious Diseases, Singapore, Singapore. Electronic address:

Published: July 2024

AI Article Synopsis

  • The Zika virus (ZIKV) is a global health concern, causing recurrent outbreaks and having the potential for widespread transmission, with serious health impacts despite many infections being asymptomatic.
  • There are no current approved vaccines or antiviral treatments for ZIKV, making the ZIKV NS2B-NS3 protease a key focus for drug development due to its essential role in the virus's life cycle.
  • This study developed a high-throughput screening assay to identify potential inhibitors of the ZIKV protease, demonstrating that it can effectively screen pharmacologically active compounds to discover new drug candidates.

Article Abstract

Zika virus (ZIKV) continues to pose a significant global public health threat, with recurring regional outbreaks and potential for pandemic spread. Despite often being asymptomatic, ZIKV infections can have severe consequences, including neurological disorders and congenital abnormalities. Unfortunately, there are currently no approved vaccines or antiviral drugs for the prevention or treatment of ZIKV. One promising target for drug development is the ZIKV NS2B-NS3 protease due to its crucial role in the virus life cycle. In this study, we established a cell-based ZIKV protease inhibition assay designed for high-throughput screening (HTS). Our assay relies on the ZIKV protease's ability to cleave a cyclised firefly luciferase fused to a natural cleavage sequence between NS2B and NS3 protease within living cells. We evaluated the performance of our assay in HTS setting using the pharmacologic controls (JNJ-40418677 and MK-591) and by screening a Library of Pharmacologically Active Compounds (LOPAC). The results confirmed the feasibility of our assay for compound library screening to identify potential ZIKV protease inhibitors.

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Source
http://dx.doi.org/10.1016/j.slasd.2024.100164DOI Listing

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