Y-27632, as a cytoskeleton protector, is commonly used for low-temperature preservation of cells. Goat sperm are prone to damage to the cytoskeleton under low-temperature conditions, leading to a loss of sperm vitality. However, the Y-27632 small molecule has not yet been used in research on low-temperature preservation of goat semen. This study aims to address the issue of low temperature-induced loss of sperm motility in goats by using Y-27632, and explore the regulation of Y-27632 on goat sperm metabolism. At a low temperature of 4 °C, different concentrations of Y-27632 were added to the sperm diluent. The regulation of Y-27632 on the quality of low temperature-preserved goat semen was evaluated by detecting goat sperm motility, antioxidant capacity, mitochondrial activity, cholesterol levels, and metabolomics analysis. The results indicated that 20 µM Y-27632 significantly increased plasma membrane integrity ( < 0.05), and acrosome integrity ( < 0.05) and sperm motility ( < 0.05), increased levels of superoxide dismutase (SOD) and catalase (CAT) ( < 0.01), increased total antioxidant capacity (T-AOC) ( < 0.05), decreased levels of malondialdehyde (MDA) and reactive oxygen species (ROS) ( < 0.01), and significantly increased mitochondrial membrane potential (MMP). The levels of ATP, Ca, and TC in sperm increased ( < 0.01). Twenty metabolites with significant differences were identified, with six metabolic pathways having a significant impact, among which the D-glutamic acid and D-glutamine metabolic pathways had the most significant impact. The artificial insemination effect of goat semen treated with 20 μM Y-27632 was not significantly different from that of fresh semen. This study indicates that Y-27632 improves the quality of low-temperature preservation of sperm by protecting the sperm plasma membrane, enhancing sperm antioxidant capacity, regulating D-glutamine and D-glutamate metabolism, and promoting the application of low-temperature preservation of semen in artificial insemination technology.
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http://dx.doi.org/10.3390/ani14101441 | DOI Listing |
Domest Anim Endocrinol
December 2024
Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China. Electronic address:
Gonadotropin-releasing hormone (GnRH) vaccines have been widely used to effectively inhibit gonadal development and reproductive function. To improve the immunogenicity of GnRH, we developed and evaluated the effects of GnRH6-kisspeptin-CRM197 immunization on the reproductive function in male goats. Thirty 3-month-old male goats (n = 30) were randomly assigned to control, surgical, and immunized groups.
View Article and Find Full Text PDFTheriogenology
December 2024
Shanghai Municipal Key Laboratory of Agri-Genetics and Breeding, Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, China; Key Laboratory of Livestock and Poultry Resources (Pig) Evaluation and Utilization, Ministry of Agriculture and Rural Affairs, Shangha, 201106, China. Electronic address:
This study examined the effect of supplementation of freezing extender with isoglycyrrhizin (ISL), a natural antioxidant agent, on the quality and fertility potential of goat spermatozoa after cryopreservation. Forty ejaculates were collected from eight Chongming White rams and diluted with five concentrations of ISL: 0 (control group), 50, 100, 150, and 200 μg/mL. The quality, motility parameters, antioxidant properties, mRNA expression of antioxidant and ferroptosis genes, and ability to induce fertilization, were evaluated following freezing/thawing.
View Article and Find Full Text PDFTheriogenology
December 2024
College of Animal Science/Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education/Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang, 550025, China. Electronic address:
During cryopreservation, spermatozoa produce excess reactive oxygen species (ROS), which attack the plasma membrane, disrupt the physiological structure of the sperm, and ultimately decrease semen quality. This study investigated the effects of different N-acetylcysteine (NAC) concentrations on the cryopreservation of semen from Qianbei Ma goats. Semen samples were collected from five bucks with motility rates above 80 %.
View Article and Find Full Text PDFAnim Reprod Sci
January 2025
Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A& F University, Yangling, Shaanxi 712100, PR China. Electronic address:
Sci Rep
November 2024
Yunnan Animal Science and Veterinary Institute, Jindian, Panlong District, Kunming, 650224, China.
Results of artificial insemination (AI) are affected by changes in sperm quality and the function throughout collection and preservation procedures. Proteome and metabolome alterations of sperm treated with the different procedures in goat, however, aren't fully understood. To this end, we sought to investigate the impacts of rectal probe electrostimulation (EE) and artificial vagina (AV) semen collection methods on the quality and the cryotolerance of goat sperm, with additional focus on proteomic and metabolomic analyses.
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