Convergent High O Affinity but Distinct ATP-Mediated Allosteric Regulation of Hemoglobins in Oviparous and Viviparous Lizards from the Qinghai-Tibet Plateau.

The functional adaptation and underlying molecular mechanisms of hemoglobins (Hbs) have primarily concentrated on mammals and birds, with few reports on reptiles. This study aimed to investigate the convergent and species-specific high-altitude adaptation mechanisms of Hbs in two lizards from the Qinghai-Tibet Plateau. The Hbs of high-altitude and were characterized by significantly high overall and intrinsic Hb-O affinity compared to their low-altitude populations. Despite the similarly low Cl sensitivities, the Hbs of high-altitude exhibited higher ATP sensitivity and ATP-dependent Bohr effects than that of , which could facilitate O unloading in respiring tissues. lizards Hbs exhibited similarly low temperature sensitivities and relatively high Bohr effects at lower temperatures, which could help to stably deliver and release O to cold extremities at low temperatures. The oxygenation properties of Hbs in high-altitude populations might be attributed to varying ratios of β2/β1 globin and substitutions on the β2-type globin. Notably, the Asn12Ala in lowland could cause localized destabilization of the E-helix in the tetrameric Hb by elimination of hydrogen bonds, thereby resulting in its lowest O affinity. This study provides a valuable reference for the high-altitude adaptation mechanisms of hemoglobins in reptiles.