AI Article Synopsis
- Scientists found a special marker called SSEA-4 that shows up on immature cells during pregnancy and is also found in a type of cancer called Ewing sarcoma.
- This marker was present on most cancer cell lines and many tumor samples, and higher levels of SSEA-4 were linked to more aggressive cancer behavior.
- T cells, which are part of our immune system, can be specially trained to target cells with SSEA-4, making it a promising way to treat this type of cancer in the future.
Article Abstract
Carbohydrate markers of immature cells during prenatal human development can be aberrantly expressed in cancers and deserve evaluation as immune targets. A candidate target in Ewing sarcoma is the globo-series ganglioside stage-specific embryonic antigen-4 (SSEA-4). We detected SSEA-4 expression on the cell surface of all of 14 EwS cell lines and in 21 of 31 (68%) primary EwS tumor biopsies. Among paired subpopulations of tumor cells with low versus high SSEA-4 expression, SSEA-4 expression was significantly and consistently associated with functional characteristics of tumor aggressiveness, including higher cell proliferation, colony formation, chemoresistance and propensity to migrate. SSEA-4 versus SSEA-4 expression was not related to expression levels of the EWSR1-FLI1 fusion transcript or markers of epithelial/mesenchymal plasticity. SSEA-4 cells selected from bulk populations regained higher SSEA-4 expression in vitro and during in vivo tumor growth in a murine xenograft model. T cells engineered to express SSEA-4-specific chimeric antigen receptors (CARs) specifically interacted with SSEA-4 positive EwS cells and exerted effective antigen-specific tumor cell lysis in vitro. In conclusion, with its stable expression and functional significance in EwS, SSEA-4 is an attractive therapeutic immune target in this cancer that deserves further evaluation for clinical translation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11126692 | PMC |
| http://dx.doi.org/10.1038/s41598-024-62849-8 | DOI Listing |
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Article Synopsis
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- T cells, which are part of our immune system, can be specially trained to target cells with SSEA-4, making it a promising way to treat this type of cancer in the future.
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