The aphidophagous gall midge, (Rondani) (Diptera: Cecidomyiidae), a dominant natural enemy of aphids, has been used as a biological control agent in many countries to control aphids in greenhouses. To identify key factors that induce diapause in , we evaluated the effects of photoperiod and temperature on the incidence of diapause in under laboratory conditions. The results showed that temperature and photoperiod had significant impacts on development and diapause in Low temperatures and a short photoperiod inhibited development, while high temperatures and a long photoperiod promoted development. Temperatures above 20 °C and a photoperiod greater than 14 h prevented diapause in . However, the highest diapause rate was recorded at under 15 °C and 10L:14D photoperiod conditions. At 15 °C, the first to third larvae were sensitive to a short photoperiod at any stage, and a short photoperiod had a cumulative effect on diapause induction. The longer the larvae received short light exposure, the higher the diapause rate appeared to be. Transcriptome sequencing analysis at different stages of diapause showed that differentially expressed genes were mainly enriched in the glucose metabolism pathway. Physiological and biochemical analyses showed that diapausing reduced water content; accumulated glycogen, trehalose, sorbitol, and triglycerides; and gradually reduced trehalose and triglyceride contents in the body with the extension of diapause time. Glycogen may be used as a source of energy, but sorbitol is usually used as a cryoprotectant. This study provided results on aspects of diapause in , providing data and theoretical support for promoting its commercial breeding and in-depth research on the molecular mechanisms underlying diapause regulation.
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http://dx.doi.org/10.3390/insects15050299 | DOI Listing |
Insects
December 2024
Department of Biology, National Museum of Natural Science, 1 Kuan-Chien Road, Taichung 404, Taiwan.
In the present study, we investigated the possible correlation between insulin/ecdysone signaling and chilling-induced egg diapause termination in . Changes in () and () gene expression levels in chilled eggs (whose diapause had been terminated by chilling to 5 °C for 90 days) exhibited no significant increase after being transferred to 25 °C, which differed from both non-diapause eggs and HCl-treated eggs. We further compared the differential temporal expressions of (, -, and ), ( and ), and ( () and ()) as well as () genes between chilled eggs and eggs kept at 25 °C.
View Article and Find Full Text PDFComp Biochem Physiol Part D Genomics Proteomics
December 2024
Department and Graduate Institute of Aquaculture, National Kaohsiung University of Science and Technology, Kaohsiung City, Taiwan. Electronic address:
This study investigated the mechanisms by which high salinity conditions stimulate adult Artemia females to produce diapaused cysts. We used a H NMR-based metabolomic approach to elucidate the metabolic regulation between ovoviviparity and oviparity in Artemia exposed to different salinities. At a salinity of 80 ppt, 100 % of females produced diapaused cysts, compared to 20 % at 50 ppt.
View Article and Find Full Text PDFEcotoxicol Environ Saf
January 2025
Department of Applied Biosciences, Kyungpook National University, Daegu 41566, South Korea; Department of Integrative Biology, Kyungpook National University, Daegu 41566, South Korea. Electronic address:
Phosphine (PH) fumigation is widely used to control insect pests in stored products globally. However, intensive PH use has led to the emergence of significant resistance in target insects. To address this issue, this study investigated PH resistance mechanisms by conducting both qualitative and quantitative proteomic analyses on the whole proteome of a PH-resistant Tribolium castaneum strain (AUS-07) using LC-MS/MS.
View Article and Find Full Text PDFCancer Res
January 2025
Medical Department of Hematology, Oncology and Tumor Immunology, Molekulares Krebsforschungszentrum - MKFZ, Campus Virchow Klinikum, Charité - Universitätsmedizin, Berlin, Germany.
Arch Insect Biochem Physiol
December 2024
Department of Sericultural Science, College of Animal Science, South China Agricultural University, Guangzhou, China.
Instant and refrigerated acid soaking are commonly used in cocoon production to prevent or break diapause, and provide developable silkworm eggs for sericulture, while their mechanisms have not been fully understood. This study aims to investigate the mechanisms by which hydrochloric acid (HCl) or dimethyl sulfoxide (DMSO) promotes embryonic development in silkworm Bombyx mori, focusing on the chloride ion (Cl) related gene expression profiles. Our results revealed that the HCl treatment of up to 6 min enhanced hatchability in freshly picked and cold-stored eggs, whereas a slight decrease in hatchability was observed in those treated with DMSO for 40 min.
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