AI Article Synopsis
- The study focuses on cloning and analyzing resistance-related genes in cucumbers to improve molecular breeding for disease resistance.
- Two specific Rho-related guanosine triphosphatases (ROP) genes in cucumbers were identified and characterized through various technical methods, including RT-qPCR and VIGS.
- Findings suggest that the cloned genes may enhance cucumber resistance against diseases by interacting with signaling pathways, specifically the ROS and ABA pathways.
Article Abstract
The cloning of resistance-related genes / and the analysis of their mechanism of action provide a theoretical basis for the development of molecular breeding of disease-resistant cucumbers. The structure domains of two Rho-related guanosine triphosphatases from plant (ROP) genes were systematically analyzed using the bioinformatics method in cucumber plants, and the genes (Cucsa.322750) and (Cucsa.197080) were cloned. The functions of the two genes were analyzed using reverse-transcription quantitative PCR (RT-qPCR), virus-induced gene silencing (VIGS), transient overexpression, cucumber genetic transformation, and histochemical staining technology. The conserved elements of the CsROP5/CsROP10 proteins include five sequence motifs (G1-G5), a recognition site for serine/threonine kinases, and a hypervariable region (HVR). The knockdown of through VIGS affected the transcript levels of ABA-signaling-pathway-related genes (, , , and ), ROS-signaling-pathway-related genes ( and ), and defense-related genes ( and ), thereby improving cucumber resistance to . Meanwhile, inhibiting the expression of regulated the expression levels of ROS-signaling-pathway-related genes ( and ) and defense-related genes ( and ), thereby enhancing the resistance of cucumber to . Overall, and may participate in cucumber resistance to through the ROS and ABA signaling pathways.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11117962 | PMC |
| http://dx.doi.org/10.3390/biology13050308 | DOI Listing |
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