AI Article Synopsis
- - The T-TAS system, which analyzes how platelets contribute to blood clotting, struggles with low platelet counts, prompting the introduction of a haemodilution chip designed for such conditions.
- - Blood samples were created using red blood cell products and platelet products, specifically tested for platelet counts of 50 × 10/μL, and various aggregation tests were conducted with different inducers like ADP.
- - The study found that the new HD chip reliably assessed haemostatic functions at low platelet counts, showing significant correlations with certain aggregation measures and suggests potential for future in vivo testing.
Article Abstract
Background And Objectives: The total thrombus-formation analysis system (T-TAS) can quantitatively analyse the contribution of platelets to haemostasis using reconstituted blood samples. However, it is unsuitable in cases with low platelet counts. We introduced a haemodilution (HD) chip with a shallow chamber depth, adapted to low platelet counts and high shear conditions (1500 s).
Materials And Methods: Blood samples were prepared by mixing red blood cell products, standard human plasma and platelet products; the final platelet count was 50 × 10/μL. Aggregation tests were performed by using the aggregation inducers collagen, adenosine diphosphate (ADP) and ristocetin. Samples with 2-, 4- and 9-day-old platelet products (N = 10) were evaluated.
Results: The HD chip enabled the stable analysis of the haemostatic function of all samples at a platelet count of 50 × 10/μL. Haemostatic function was correlated with ADP aggregation (time to 10 kPa [T]: r = -0.53; area under the curve for 30 min: r = 0.40) and storage period (T: r = 0.44).
Conclusion: The HD chip-mounted T-TAS can stably analyse haemostatic function under low platelet counts and high shear conditions; this approach is expected to serve as a bridge to in vivo haemostatic tests with experimental animals.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/vox.13683 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Predictive value of inflammatory indices for one year outcome of primary percutaneous coronary intervention on saphenous vein graft in post-coronary artery bypass grafting patients.
Acta Cardiol
January 2025
Cardiovascular Intervention Research Center, Rajaie Cardiovascular, Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran.
Background: This study aims to evaluate the prognostic value of the inflammatory indices i.e. Platelet Lymphocyte Ratio (PLR), Neutrophil Lymphocyte Ratio (NLR), and Systemic Immune Inflammation Index (SII) as potential predictors of Major Adverse Cardiovascular Event (MACE) in patients undergoing Primary Percutaneous Coronary Intervention (PPCI) on Saphenous Vein Graft (SVG) with a history of Coronary Artery Bypass Graft (CABG).
View Article and Find Full Text PDFPyrazinyl-Substituted Aminoazoles as Covalent Inhibitors of Thrombin: Synthesis, Structure, and Anticoagulant Properties.
ACS Pharmacol Transl Sci
January 2025
Institute of Pharmaceutical and Medicinal Chemistry, University of Münster, 48149 Münster, Germany.
This study presents a novel series of -acylated 1,2,4-triazol-5-amines and 1-pyrazol-5-amines, featuring a pyrazin-2-yl moiety, developed as covalent inhibitors of thrombin. These compounds demonstrated potent inhibitory activity, with derivatives and achieving IC values as low as 0.7 and 0.
View Article and Find Full Text PDFMiddle-throughput LC-MS-based platelet proteomics with minute sample amounts using semi-automated positive pressure FASP in 384-well format (PF384).
Thromb Haemost
January 2025
Department of Bioinformatics, Biocenter, University of Würzburg, Wurzburg, Germany.
Comprehensive characterization of platelets requires various functional assays and analysis techniques, including omics-disciplines, each requiring an individual aliquot of a given sample. Consequently, the sample material per assay is often highly limited rendering downscaling a prerequisite for effective sample exploitation. Here we present a transfer of our recently introduced 96-well-based proteomics workflow (PF96) into the 384-well format (PF384) allowing for a significant increase in sensitivity when processing minute platelet protein amounts.
View Article and Find Full Text PDFα-Actinin-1 deficiency in megakaryocytes causes low platelet count, platelet dysfunction, and mitochondrial impairment.
Blood Adv
January 2025
The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Cytoskeletal remodeling and mitochondrial bioenergetics play important roles in thrombocytopoiesis and platelet function. Recently, α-actinin-1 mutations have been reported in patients with congenital macrothrombocytopenia. However, the role and underlying mechanism of α-actinin-1 in thrombocytopoiesis and platelet function remain elusive.
View Article and Find Full Text PDFPeripheral blood biomarkers as differential diagnostic markers of disease severity in neonates with hyperbilirubinemia.
Heliyon
January 2025
Department of Hematology and Immunohematology, School of Biomedical and Laboratory Sciences, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.
Background: Blood biomarkers offers an independent insight for the pathophysiology of hyperbilirubinemia. However, they are not practically used for the differential diagnosis of the hyperbilirubinemia severity. Therefore, the current study aimed to assess the differential diagnostic value of peripheral blood biomarkers with disease severity as an alternative.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!