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Characterization and validation of TaAGL66, a gene related to fertility conversion of wheat in the presence of Aegilops kotschyi cytoplasm. | LitMetric

AI Article Synopsis

  • TaAGL66 is a MADS-box transcription factor found in wheat that is important for anther and pollen development, influencing male fertility, particularly in the context of Aegilops kotschyi cytoplasm.
  • The study identified this gene through RNA sequencing and expression analysis, confirming its high expression specifically in fertile anthers and its nuclear localization.
  • Silencing TaAGL66 led to significant reductions in fertility, abnormal anther behavior, and poor pollen development, highlighting its critical role in achieving male fertility in these wheat varieties.

Article Abstract

TaAGL66, a MADS-box transcription factor highly expressed in fertile anthers of KTM3315A, regulates anther and/or pollen development, as well as male fertility in wheat with Aegilops kotschyi cytoplasm. Male sterility, as a string of sophisticated biological processes in higher plants, is commonly regulated by transcription factors (TFs). Among them, MADS-box TFs are mainly participated in the processes of floral organ formation and pollen development, which are tightly related to male sterility, but they have been little studied in the reproductive development in wheat. In our study, TaAGL66, a gene that was specifically expressed in spikes and highly expressed in fertile anthers, was identified by RNA sequencing and the expression profiles data of these genes, and qRT-PCR analyses, which was localized to the nucleus. Silencing of TaAGL66 under fertility condition in KTM3315A, a thermo-sensitive male sterile line with Ae. kotschyi cytoplasm, displayed severe fertility reduction, abnormal anther dehiscence, defective pollen development, decreased viability, and low seed-setting. It can be concluded that TaAGL66 plays an important role in wheat pollen development in the presence of Ae. kotschyi cytoplasm, providing new insights into the utilization of male sterility.

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Source
http://dx.doi.org/10.1007/s00425-024-04416-zDOI Listing

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