AI Article Synopsis
- - The study explored the potential of using a specific nitrogen fixation gene cluster (VNnif) from certain microorganisms as an alternative to chemical fertilizers, focusing on its impact on plant growth.
- - Researchers improved the nitrogenase activity of the plant growth-promoting rhizobacteria CHA0, achieving enhanced efficiency through genetic modifications, resulting in 48.16 nmol CH/mg/h.
- - The engineered bacteria CHA0-PVNnif demonstrated positive effects on the growth of specific plants, contributing to the understanding and application of microbial solutions for sustainable agriculture.
Article Abstract
Microbial nitrogen fixation presents a viable alternative to chemical fertilizers, yet the limited colonization and specificity of naturally occurring nitrogen-fixing microorganisms present significant challenges to their widespread application. In this study, we identified a nitrogen fixation gene cluster (VNnif) in (VN) and tested its nitrogenase activity through the acetylene reduction assay. We investigated the potential utilization of nitrogenase by incorporating the nitrogenase gene cluster from VN into plant growth-promoting rhizosphere bacteria CHA0 and enhancing its activity to 48.16 nmol CH/mg/h through promoter replacement and cluster rearrangement. The engineered strain CHA0-PVNnif was found to positively impact the growth of col-0 and L. (wheat). This study expanded the role of plant growth-promoting rhizobacteria (PGPR) and provided a research foundation for enhancing nitrogenase activity.
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| http://dx.doi.org/10.1021/acs.jafc.4c01232 | DOI Listing |
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