AI Article Synopsis
- Cervical cancer is a major health issue for women, ranking fourth in incidence and mortality, with the RNA-binding protein YTHDF2 playing a role in its progression through unclear mechanisms.
- * Researchers conducted various assays to study the effects of knocking down YTHDF2 on cervical cancer cells, finding that it influences tumor stemness and apoptosis.
- * Data analysis suggested that YTHDF2 regulates GLI2, leading to increased JNK activity and endoplasmic reticulum stress, which ultimately suppresses cervical cancer cell growth and highlights YTHDF2 as a potential therapeutic target.
Article Abstract
Cervical cancer ranks fourth in women in terms of incidence and mortality. The RNA-binding protein YTH N6-methyladenosine RNA-binding protein F2 (YTHDF2) contributes to cancer progression by incompletely understood mechanisms. We show how YTHDF2 controls the fate of cervical cancer cells and whether YTHDF2 could be a valid target for the therapy of cervical cancer. Sphere formation and alkaline phosphatase staining assays were performed to evaluate tumor stemness of cervical cancer cells following YTHDF2 knockdown. Apoptosis was detected by flow cytometry and TUNEL assay. The compounds 4PBA and SP600125 were used to investigate the correlation between JNK, endoplasmic reticulum stress, tumor stemness, and apoptosis. Data from The Cancer Genome Atlas (TCGA) databases and Gene Expression Omnibus (GEO) revealed that GLI family zinc finger 2 (GLI2) might be the target of YTHDF2. The transcription inhibitor actinomycin D and dual-luciferase reporter gene assays were employed to investigate the association between the GLI2 mRNA and YTHDF2. Nude mouse xenografts were generated to assess the effects of YTHDF2 knockdown on cervical cancer growth in vivo. Knockdown of YTHDF2 up-regulated the expression of GLI2, leading to JNK phosphorylation and endoplasmic reticulum stress. These processes inhibited the proliferation of cervical cancer cells and their tumor cell stemness and promotion of apoptosis. In conclusion, the knockdown of YTHDF2 significantly affects the progression of cervical cancer cells, making it a potential target for treating cervical cancer.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11141453 | PMC |
| http://dx.doi.org/10.1016/j.tranon.2024.101994 | DOI Listing |
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