The anti-fatigue and sleep-aiding effects vary significantly among different recipes containing extracts.

Aims: This study aims to delve into the anti-fatigue and sleep-aiding effects of various formulations containing extracts.

Materials And Methods: PGB [incorporating extract (GE), broken spore powder (GB) and mycelium (PH)] and GBS [composed of GE, GB, and powder (GS)] were chosen as representative recipes for this study. Mice were treated with these recipes or key components of for 14 consecutive days. Subsequently, a weight-bearing swimming experiment was conducted to assess the mice's exhaustion time and evaluate the anti-fatigue properties of the recipes. Sleep-aiding effects were analyzed by measuring the sleep latency and duration. Furthermore, levels of blood lactic acid, serum urea nitrogen, hepatic glycogen, muscle glycogen, and malondialdehyde (MDA) were measured in the livers and muscles.

Key Findings: The anti-fatigue abilities of the tested mice were significantly improved after treatment with PGB and their sleep quality improved as well with GBS treatment. PGB treatment for 14 days could significantly prolong the exhaustion time in weight-bearing swimming (from 10.1 ± 0.5 min to 15.2 ± 1.3 min). Meanwhile, glycogen levels in the livers and muscles were significantly increased, while the levels of serum lactic acid, serum urea nitrogen, and MDA in the livers and muscles were significantly decreased. In contrast, mice treated with GBS for 14 days experienced significant improvements in sleep quality, with shortened sleep latency (from 6.8 ± 0.7 min to 4.2 ± 0.4 min), extended sleep duration (from 88.3 ± 1.4 min to 152.5 ± 9.3 min), and decreased muscle MDA levels. These results indicated that extracts can be used for anti-fatigue and or aid in sleeping, depending on how they are prepared and administered.

Significance: This study provides experimental evidence and theoretical basis for the development of recipes that are specifically designed to help with anti-fatigue and sleep.