AI Article Synopsis
- Osteomyelitis is a complex bone infection that needs better understanding of its immune and molecular mechanisms; this study looks into how cuproptosis-related genes (CRGs) play a role in this context.
- The researchers analyzed public transcriptomic datasets to identify consistent expression patterns of CRGs in infected osteomyelitis, focusing on M2 macrophage-related genes, and performed various analyses to uncover their potential mechanisms.
- They identified three key CRGs (SLC31A1, DLD, and MTF1), which differ in their immune responses, and developed a diagnostic model for osteomyelitis based on these genes, validating their findings with additional data.
Article Abstract
Objective: Osteomyelitis is a challenging disease in the field of bone infections, with its immune and molecular regulatory mechanisms still poorly understood. The aim of this study is to explore the value and potential mechanisms of cuproptosis-related genes (CRGs) in ()-infected osteomyelitis from an immunological perspective.
Methods: Initially, three transcriptomic datasets from public databases were integrated and analyzed, and consistent expression of CRGs in -infected osteomyelitis was identified. Subsequently, immune infiltration analysis was performed, and M2 macrophage-related CRGs (M2R-CRGs) were further identified. Their potential molecular mechanisms were evaluated using Gene Set Variation Analysis (GSVA) and Gene Set Enrichment Analysis (GSEA). Finally, distinct osteomyelitis subtypes and diagnostic models based on characteristic M2R-CRGs were constructed.
Results: Through correlation analysis with immune cell infiltration, three characteristic M2R-CRGs (SLC31A1, DLD, and MTF1) were identified. Further analysis using unsupervised clustering and immune microenvironment analysis indicated that cluster 1 might activate pro-inflammatory responses, while cluster 2 was shown to exhibit anti-inflammatory effects in osteomyelitis. Compared to Cluster A, Cluster B demonstrated higher levels and a greater diversity of immune cell infiltrations in CRG-related molecular patterns, suggesting a potential anti-inflammatory role in osteomyelitis. A diagnostic model for -infected osteomyelitis, based on the three M2R-CRGs, was constructed, exhibiting excellent diagnostic performance and validated with an independent dataset. Significant upregulation in mRNA and protein expression levels of the three M2R-CRGs was observed in rat models of -infected osteomyelitis, aligning with bioinformatic results.
Conclusion: The M2R-CRGs (SLC31A1, DLD, and MTF1) may be considered characteristic genes for early diagnosis and personalized immune therapy in patients with -infected osteomyelitis.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11104443 | PMC |
| http://dx.doi.org/10.2147/JIR.S457414 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Oral vs Intravenous Antibiotics for Fracture-Related Infections: The POvIV Randomized Clinical Trial.
JAMA Surg
January 2025
Vanderbilt University Medical Center, Nashville, Tennessee.
Importance: Fracture-related infection (FRI) is a serious complication following fracture fixation surgery. Current treatment of FRIs entails debridement and 6 weeks of intravenous (IV) antibiotics. Lab data and retrospective clinical studies support use of oral antibiotics, which are less expensive and may have fewer complications than IV antibiotics.
View Article and Find Full Text PDFEvaluation and analysis of efficacy in bisphosphonate treatment of chronic nonbacterial osteomyelitis.
Hua Xi Kou Qiang Yi Xue Za Zhi
February 2025
State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases & Dept. of Pharmacy, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China.
Objectives: This study aimed to analyze the influence of drug factors on the efficacy of bisphosphonate for chronic nonbacterial osteomyelitis to provide a reference for clinical treatment and promote clinical rational drug use by evaluation of effectiveness and safety of bisphosphonate treatment of chronic nonbacterial osteomyelitis.
Methods: Literature on the treatment of chronic nonbacterial osteomyelitis by using bisphosphonate was collected and analyzed from PubMed, Medline, Embase, Cochrane, ISI Web of Knowledge, CNKI, VIP, and Wanfang databases.
Results: A total of 489 cases were collected, with an average complete response rate of clinical presentation, laboratory tests and imaging findings of 80.
Case report: A novel germline loss-of-function mutation in the STAT1 transactivation domain in two Chinese siblings, with the elder sibling presenting with multifocal Calmette-Guerin osteomyelitis.
Front Immunol
January 2025
Department of Paediatrics and Adolescent Medicine, Queen Mary Hospital, Hong Kong, Hong Kong SAR, China.
Signal transducer and activator of transcription 1 (STAT1) gene mutations have broad clinical phenotypes, classified by the inheritance pattern and functional state. Individuals with autosomal dominant STAT1 deficiency are more susceptible to intracellular bacteria, the hallmark of which is Mendelian susceptibility to mycobacterial diseases (MSMDs) that are associated with increased risks of invasive disease by weakly virulent mycobacteria. We report a novel heterozygous missense mutation in exon 23 of the STAT1 gene (NM_007315.
View Article and Find Full Text PDFAdjunctive Hyperbaric Oxygen Therapy for Patients with Chronic Refractory Osteomyelitis: A Report of Treatment Outcomes and Risk Factors for Treatment Failure.
Adv Skin Wound Care
January 2025
At the University of South Carolina, Columbia, South Carolina, USA, J. Benjamin Jackson III, MD, MBA, is Professor of Orthopaedic Surgery and Director of Orthopaedic Research, Department of Orthopaedics; and Yianni Bakaes, BS, and Ben Jacques, BS, are Medical Students, School of Medicine. Chase Gauthier, MD, is Research Fellow, Prisma Health Department of Orthopedics, Columbia, South Carolina. Also at the University of South Carolina, William L. Mills Jr, MD, and Kenny Nguyen, MD, are Medical Residents, School of Medicine; Tyler Gonzalez, MD, MBA, is Assistant Professor of Orthopaedic Surgery, Department of Orthopaedics; and David L. Cone, MD, is Clinical Associate Professor of Family and Preventative Medicine, School of Medicine. The authors have disclosed no financial relationships related to this article. Submitted September 6, 2023; accepted in revised form January 16, 2024.
Objective: To evaluate the effect of hyperbaric oxygen (HBO) therapy on the outcomes of patients with chronic refractory osteomyelitis (CRO) when combined with modern antibiotics with modern delivery methods and/or surgical treatments.
Methods: The authors conducted a retrospective review on 58 patients with CRO from a single institution who underwent HBO therapy along with standard treatment between January 2009 and December 2019. To investigate associations with binary outcomes of interest, they estimated logistic regression models.
Vertebral osteomyelitis in patients with infective endocarditis: prevalence, risk factors and mortality.
Eur J Clin Microbiol Infect Dis
January 2025
Department of Cardiology, Noordwest Hospital, Alkmaar, The Netherlands.
Purpose: Infective endocarditis (IE) can be complicated by vertebral osteomyelitis (VO). This study investigates risk factors associated with VO in patients with infective endocarditis, and 6-month mortality and relapse rates in patients with IE and concomitant VO.
Methods: We performed a observational study in two hospitals between September 2016 and October 2022.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!