AI Article Synopsis
- The study presents two new synthetic riboswitches designed to respond to specific molecules, ASP2905 and theophylline.
- These riboswitches were integrated into a cell-free protein synthesis (CFPS) system that was encapsulated in liposomes.
- External stimulation using the corresponding ligands allowed for precise control over gene expression regulated by the riboswitches.
Article Abstract
Here we report two novel synthetic riboswitches that respond to ASP2905 and theophylline and function in reconstituted cell-free protein synthesis (CFPS) system. We encapsulated the CFPS system as well as DNA-templated encoding reporter genes regulated by these orthogonal riboswitches inside liposomes, and achieved switchable and orthogonal control over gene expression by external stimulation with the cognate ligands.
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| http://dx.doi.org/10.1039/d4cc00965g | DOI Listing |
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