Effects of temperature and nutrients on growth and toxicity of Alexandrium affine from southeastern Gulf of California.

An Alexandrium affine strain (AAJQ-1) from San José Island, Gulf of California was characterized for growth and toxicology. Fivefold of f/2 + Se cultures were incubated for 34 days in a temperature gradient (21-29 °C). Aliquots were collected every third day for cell counting, toxin determination, and nutrient analyses. In this study ELISA method was used to evaluate the PSP toxin production due to the lower detection limit than the HPLC method. The highest cell density (6724 cells mL) and growth rate (0.22 day) were obtained at 27 °C and they were related to temperature in all treatments. Cell density showed negative correlation with nitrate at temperatures ≥23 °C, and with orthophosphate 27 °C, furthermore, these correlations promote the toxin production (0.05-0.45 fmol STX cell); beyond that nitrite at high temperature seems to promote toxin production, which has not been sufficiently documented.